- Make solution of HRP-GNPs using beta method
- Make Hemoglobin-GNP solution and HRP-GNP solution using alpha method
Monitoring Activity of HRP-AuNP complexes
- HRP-AuNP complex solution from the alpha method and the polydopamine-coated AuNPs were placed in high velocity tubes.
- The nano particle solutions were spun at 18,000rpm for 3 hours.
- The supernatants were discarded and the pellets were resuspended in water.
- The polydopamine-coated AuNPs were stored at 4 degrees Celsius
- The resuspended HRP-AuNP complex solutions were analyzed for activity using UV-Vis
- The nano particles were added to hydrogen peroxide and OPD (Ophenylenediamine)
- Active HRP catalyzes the reaction of Ophenylenediamine with H2O2 to produce 2,3-Diaminophenazine
- Stock solution of OPD with a concentration of 1mg/mL
- Stock solution of H2O2 113uL
OPD and H2O2 UV/Vis
HRP supernatant UV/Vis
- Series are at 10 minute intervals, starting at 0