User:Helen L. Slucher/Notebook/CHEM-572/2014/01/29

From OpenWetWare
Jump to navigationJump to search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


  1. Prepare stock solutions
    1. Dissolve HAuCl4 in water to have a solution of 10mM
    2. Dissolve 250mg of the citrate sodium salt into 25mL of water
  2. In a beaker mix 50mL of water and a volume of the gold stock solution
  3. Bring solution to a boil
  4. Add a volume of the citrate sodium solution and reflux for 30 minutes
    1. Note color change from dark blue to red
  • Different solutions
    1. 1.236mL gold and 0.509mL citrate
    2. 2.5mL gold and 0.509mL citrate
    3. 2mL gold and 0.509mL citrate
    4. 2mL gold and 1.5mL citrate
    5. 2mL gold and 2.5mL citrate
    6. 6mL gold and 1.5mL citrate
    7. 2mL gold and 4mL citrate


The UV-Vis spectra of the solutions were obtained and the following graph represents the spectra: UV-Vis of Nanoparticle Solutions Danelmen.png

The UV-Vis data allowed to calculate particle size using this reference. The following table summarizes those results and the calculated size of the particles for each solution.

Solution no. 1 2 3 4 5 6 7
Aspr 0.146 0.29 0.268 0.191 0.294 0.507 0.29
A450 0.082 0.146 0.145 0.116 0.196 0.313 0.182
Aspr/450 1.780487805 1.98630137 1.848275862 1.646551724 1.5 1.619808307 1.593406593
Size (nm) 20-25 40-45 25-30 14-16 10 14-16 12.0-14.0

Solutions 1 and 5 were the only two not to form gold precipitate during nano particle synthesis. From the results, solution yields the best nano particle size and that ratio will be used throughout the semester for nano particle synthesis.