User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/02

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  1. Run fluorescence data for lysozyme at pH=10
  2. Create stock solutions for use on 11.08.2016



  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 11/02/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.


Figure 1 Maximum emission data in a solution of pH 10.

Figure 2 Integrated fluorescence data in a solution of pH 10.

Figure 3 Full emission spectra for important time points: t=0, 12, 42, 96, 168 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=12 min, this maximum has shifted up and to the right.
  3. Until t=42 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=42 min, the maximum begins increasing again.
  5. At t=96 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=168 shows the final full spectra obtained.

UV-vis solutions for 11.08.2016