Objectives
- Run fluorescence data for lysozyme at pH=10
- Create stock solutions for use on 11.08.2016
Description
Fluorescence
- Solutions made using measurements below
- Fluorescence data run every 3 minutes for 3 hours
Solutions for 11/02/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.
Data
Figure 1 Maximum emission data in a solution of pH 10.
Figure 2 Integrated fluorescence data in a solution of pH 10.
Figure 3 Full emission spectra for important time points: t=0, 12, 42, 96, 168 min.
Figure 3 Explanation
- At t=0 min, we see a max emission far to the left of the other emission spectra.
- At t=12 min, this maximum has shifted up and to the right.
- Until t=42 min, the maximum for each spectra decreases until the shown maximum.
- After t=42 min, the maximum begins increasing again.
- At t=96 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
- t=168 shows the final full spectra obtained.
UV-vis solutions for 11.08.2016
|
Project name
Main project page
Previous entry Next entry
