User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/25

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Objectives Run fluorescence data for lysozyme at pH=9 Create stock solutions for use on 10.26.2016 Description Fluorescence Solutions made using measurements below Fluorescence data run every 3 minutes for 3 hours Solutions for 10/05/2016 Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours. Data Figure 1 Maximum emission data in a solution of pH 9. Figure 2 Integrated fluorescence data in a solution of pH 7. Figure 3 Full emission spectra for important time points: t=0, 6, 33, 90, 180 min. Figure 3 Explanation At t=0 min, we see a max emission far to the left of the other emission spectra. At t=6 min, this maximum has shifted up and to the right. Until t=33 min, the maximum for each spectra decreases until the shown maximum. After t=33 min, the maximum begins increasing again. At t=90 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away. t=180 shows the final full spectra obtained. UV-vis solutions for 10.26..2016 Table 1 UV-vis solutions made for [Fructose]=.75mM with silver Table 2 UV-vis solutions made for [Fructose]=1.25mM with silver