- Gareth (Gary) Trubl, Ph.D.
- Livermore, CA 94550
- Trubl.1@OSU.edu or Trubl1@llnl.gov
- Scopus Author ID: 56178224700
- Web of Science ResearcherID: L-7977-2019
- Twitter: @gtrubl
I am a proud father and husband. I have always had a love for the environment and have been interested in how science and policy can shape our future. I love outreach and believe it is our duty as scientists to shape the next generation. I am currently interested in viruses that infect bacteria and archaea in soil systems. Previously this was limited to naked dsDNA viruses, but has expanded to include ssDNA viruses and hopefully soon RNA viruses.
Postdoc at Lawrence Livermore National lab
Using stable isotope probing and nano-secondary ion mass spectroscopy techniques with viral metagenomes (SIP-Viromics) to characterize soil viruses and virus-host linkages. I want to understand how wet-up influences phage-induced mortality and carbon cycling.
- 2015–Dec. 2018, Ph.D. in Microbiology, The Ohio State University
- Advisers Drs. Virginia Rich and Matthew Sullivan
half-time 1/15–8/15, for medical leave, birth of child, & lab relocation
- 2013–2015, Ph.D. in Soil, Water, and Environmental Science, University of Arizona
- 2011–2013, M.S. in Environmental Science and Health, University of Nevada, Reno/Desert Research Institute
- Adviser Dr. Alison Murray
- 2007–2011, B.S. in Environmental Microbiology, University of Arizona
- Minor in Chemistry
- Adviser Dr. Peter Cotty
Microbial Ecology, Polar Microbiology, Environmental Science, Biogeochemistry, Astrobiology, Geobiology, Environmental Microbiology, Soil Microbiology, Meta-omics, Climate Change, and Biotechnology
- Wetting induces viral blooms in biological soil crust (submitted to mBio). Van Goethem M.W., Swenson T.L., Trubl G., Roux S., & Northen T.R.
- Towards optimized viral metagenomes for double-stranded and single-stranded DNA viruses from challenging soils (2019). Trubl G., Roux S., Solonenko N., Li Y-F., Bolduc B., Rodríguez-Ramos J., Eloe-Fadrosh E.A., Rich V.I., & Sullivan M.B. PeerJ 7:e7265
- Optimizing de novo genome assembly from PCR-amplified metagenomes (2019). Roux S., Trubl G., Goudeau D., Nath N., Couradeau E., Ahlgren N.A., Zhan Y., Marsan D., Chen F., Fuhrman J.A., Northen T.R., Coleman M.L., Sullivan M.B., Rich V.I., Malmstrom R.R., & Eloe-Fadrosh E.A. PeerJ 7:e6902.
- Pioneering Soil Viromics to Elucidate Viral Impacts on Soil Ecosystem Services (2018; PhD dissertation). Trubl G.The Ohio State University; osu1543425468999981
- Soil viruses are underexplored players in ecosystem carbon processing (2018). Trubl G., Jang H-B., Roux S., Emerson J.B., Bolduc B., Solonenko N., Ellenbogen J., Runyon A.T., Vik D.R., Solden L., Woodcroft B.J., Tyson G.W., Wrighton K.C., Saleska S.R., Sullivan M.B., & Rich V.I. mSystems, 3(5), pp.e00076-18.
*Part of figure 4 was a featured image in volume 3, issue 5
- Host-linked soil viral ecology along a permafrost thaw gradient (2018). Emerson J.B., Roux S., Brum J.R., Bolduc B., Woodcroft B.J., Jang H-B., Singleton C.M., Solden L.M., Naas A.E., Boyd J.A., Hodgkins S.B., Wilson R.M., Trubl G., Li C., Frolking S., Pope P.B., Wrighton K.C., Crill P.M., Chanton J.P., Saleska S.R., Tyson G.W., Rich V.I., & Sullivan M.B. Nature Microbiology, 3(8), p.870.
- Chemodenitrification in the cryoecosystem of Lake Vida, Victoria Valley, Antarctica (2016). Ostrom N.E., Gandhi H., Trubl G. and Murray A.E. Geobiology, 14(6), pp.575-587.
- Optimization of viral resuspension methods for carbon-rich soils along a permafrost thaw gradient (2016). Trubl G., Solonenko N., Chittick L., Solonenko S.A., Rich V.I., Sullivan M.B. PeerJ 4:e1999.
- Illuminating structural proteins in viral “dark matter” with metaproteomics (2016). Brum J.R., Ignacio-Espinoza J.C., Kim E-H., Trubl G., Jones R.M., Roux S., VerBerkmoes N.C., Rich V.I. and Sullivan M.B., 2016. Proceedings of the National Academy of Sciences, 113(9), pp.2436-2441.
- Mesquite pods: A superfood that is delicious, free, and right in your backyard (2014). Trubl G.. Tucson, AZ: The University of Arizona.
- Brine Assemblages of Ultrasmall Microbial Cells within the Ice Cover of Lake Vida, Antarctica (2014). Kuhn E., Ichimura A.S., Peng V., Fritsen C.H., Trubl G., Doran P.T., Murray A.E. Appl. Environ. Microbiol, 80 (12) 3687-3698.
- Insights into the origin of N2O in Lake Vida brine (2013, MS thesis). Trubl G. University of Nevada, Reno, 64; 1545738.
- Evergreen Phage Meeting 2019
- DOE IsoGenie consortium meeting 2019
- University of California, Berkeley, Firestone lab 2019
- Joint Genome Institute Metagenome Program 2018
- Lawrence Berkeley National Lab BioSciences annual meeting 2018
- American Geophysical Union (AGU) 2017
- OSU Department of Microbiology symposium 2017
- DOE IsoGenie consortium meeting 2017
- Nevada Board of Regents meeting 2013
Poster presentations (conference, date, location, and authors):
- 2019 Astrobiology Science Conference (AbSciCon), June 24–28, 2019, Seattle, Washington. Trubl G., Roux S., Li Y-F., Solonenko N., Eloe-Fadrosh E., Rich V.I., and Sullivan M.B.
- Lawrence Livermore postdoc symposium, May 2019, Livermore, CA. Trubl G., Campbell A., Kimbrel J.A., Waldrop M.P., Pett-Ridge J., & Blazewicz S.J.
- VEGA, 2018, San Francisco, CA. Trubl G., Emerson J.B., Roux S., Jang H-B, Bolduc B., Solonenko N., Li Y-F., Eloe-Fadrosh E.A., Rich V.I., & Sullivan M.B.
- Midwest Geobiology Conference, 2017, IUPUI, IN. Trubl G., Roux S., Bolduc B., Jang H-B, Emerson J.B., Solonenko N., Sullivan M.B., & Rich V.I.
- AbSciCon, 2017, Mesa, AZ. Trubl G., Roux S., Emerson J.B., Bolduc B., Jang H-B, Solonenko N., Rich V.I., & Sullivan M.B.
- AGU, 2016, San Francisco, CA. Trubl G., Roux S., Jang HB., Solonenko N., Sullivan M.B., & Rich V.I.
- AGU, 2016, San Francisco, CA. *Emerson J.B., Roux S., Bolduc B., Brum J.R., Woodcroft B.J., Boyd J.A., Hodgkins S.B., Wilson R.M., Trubl G., Chanton J.P., Saleska S.R., Tyson G.W., Rich V.I., and Sullivan M.B.
- International Society for Microbial Ecology (ISME), 2016, Montréal, Canada. Trubl G., Roux S., Jang H-B., Ellenbogen J., Emerson J.B., Solonenko N., Sullivan M.B., & Rich V.I.
- ISME, 2016, Montréal, Canada. *Emerson J.B., Roux S., Bolduc B., Brum J.R., Woodcroft B.J., Boyd J.A., Hodgkins S.B., Wilson R.M., Trubl G., Chanton J.P., Saleska S.R., Tyson G.W., Rich V.I., and Sullivan M.B.
- 11th Annual DOE-JGI, Genomics of Energy & the Environment Meeting, 2016, CA. *Brum, J.R., Roux, S., Rich, V.I., Sullivan, M.B., and collaborators.
- AGU, 2014, San Francisco, CA. Trubl G., Solonenko N., Moreno M., Phu D., Sullivan M.B., & Rich V.I.
- AGU, 2014, San Francisco, CA. *Niu D., Ciscato E., Trubl G., Maldonado J.G., Berelson W.M., Johnson H.A., Stevenson B.S., Stamps B.W., Corsetti F.A., Spear J.R., and Geobiology 2014.
- UA Institute of the environment Grad Blitz, Tucson, AZ. Trubl G., Solonenko N., Moreno M., Phu D., Sullivan M.B., & Rich V.I.
- Arizona-Nevada Academy of Sciences (ANAS), 2014, Flagstaff, AZ. Kim E-H., Trubl G., Ignacio-Espinoza J.C., Jones R., VerBerkmoes N., Rich V.I., & Sullivan M.B.
- DRI Graduate Student poster competition, 2013, Reno, NV. 1st Place Trubl G., Kuhn E., Ostrom N.E., Fritsen C.H., Murray A.E. UNR * * * Graduate Student Association’s graduate poster competition, 2013, Reno, NV.Trubl G., Kuhn E., Ostrom N.E., Fritsen C.H., Murray A.E.
- AGU, 2012, San Francisco, CA.Trubl G., Kuhn E., Ichimura A.S., Fritsen C.H., Madigan M.T., Murray A.E.
- International Symposium on Isotopomers, 2012, Washington, DC. *Ostrom N.E., Murray A.E., Trubl G., Kuhn E
- ANAS, 2012, Microbial denitrification in Lake Vida cryobrine. Trubl G., Kuhn E., Ostrom N.E., Murray A.E.
- Postdoc at LLNL, Physical & Life Sciences Directorate, Livermore, California (Dec. 2018 – present)
- My research is advancing our understanding of soil viruses. Current work is applying SIP and NanoSIMS techniques with viromics to characterize soil viruses and virus-host linkages.
- Mentor: Steve Blazewicz
- Graduate Research Assistant, The Ohio State University (OSU), Columbus, Ohio (June 2015 – Dec. 2018); Previously at the University of Arizona (July 2013 – June 2015).
- Overall project focus is to quantify and predict thawing permafrost response to a changing climate, scaling from genes to ecosystem processes in Abisko, Sweden. My role is to help examine the viral ecology and the viruses’ metabolic potential and quantitatively relate it to biogeochemical fluxes
- Committee: Virginia Rich (adviser), Matthew Sullivan (co-adviser), Kelly Wrighton, and Michael Wilkins
- Graduate Research Assistant, Desert Research Institute (DRI)/University of Nevada, Reno (UNR), Nevada System of Higher Education, Reno, Nevada (Aug. 2011 – Aug. 2013)
- Project focus was to better understand the microbial diversity, biogeochemistry, and specifically the nitrogen cycle physiology of bacteria in brine from Lake Vida, Antarctica. This work :used microbial culture techniques (aerobically and anaerobically) using isolates from this habitat to then study their roles in biogeochemical cycling, with a focus on N2O production. To :do this, I screened cultivars for genes and proteins of interest, quantified their biogeochemistry and used stable isotope techniques to identify isotopomers and isotopologues of N2O to :determine the source (abiotic or biological) and pathways involved in its cycling.
- Adviser: Dr. Alison Murray
- Biological Aid, United States Department of Agriculture-Agricultural Research Service (USDA-ARS; 2009 – 2011)
- The laboratory leads aflatoxin management through improved understanding of aflatoxin-producing fungi and the aflatoxin contamination process. Responsibilities included studies of the :etiology and epidemiology of contamination as well as adaptation, divergence, dispersal, pathogenicity, morphogenesis, and cellular regulation of fungi. Experiments included (1) :extraction and analysis of B1 toxin and cyclopiazonic acid, (2) seed coating, and (3) fungal isolate diversity.
- Adviser: Dr. Peter Cotty
- Lab Technician, UA, Tree Ring Research Lab (2008)
- Received, labeled, and sorted cross sections and core samples. Sanded, analyzed, and photographed samples to support graduate student research.
- Supervisor: Dr. Ramzi Touchan
Teaching, Outreach, & other experiences
- Rylee Genner, M.S. (May 28–August 9; 11 weeks), summer ACP student at LLNL
- Lennel Camuy-Velez (Jan–March 2018; 7 weeks), graduate student, OSU, Columbus, Ohio
- Josue Ramos Rodriguez (Aug–Oct 2017; 7 weeks), graduate student, OSU, Columbus, Ohio
- Dylan Cronin (Aug–Oct 2017; 7 weeks), graduate student, OSU, Columbus, Ohio
- Emma Hans (Sept 2016–June 2017), high school senior, OSU, Columbus, Ohio
- Alex Runyon (Aug–Oct 2016; 7 weeks), graduate student, OSU, Columbus, Ohio
- Jared Ellenbogen (Aug–Oct 2015; 7 weeks), graduate student, OSU, Columbus, Ohio
- David Phu (Sept–Dec 2014), undergraduate student, UA, Tucson, Arizona
- Darya Anderson, Krystalle S. Diaz, Maya Sederholm, & Morgan O. Binder (full-time Jan.–April 2014 for poster preparation and presentation at The Arizona-Nevada Academy of Sciences meeting; sporadically May 2014–Dec. 2014)
- My proposal "Pioneering soil viromics to elucidate virus impacts on soil ecosystem services" was funded to characterize 20 deeply-sequenced viromes. The viromes were generated in fall 2017 and the fellowship period was used to bioinformatically process the viromes.
- 2016 Santander Summer School Scholar, Universidad Internacional Menéndez Pelayo (UIMP), Palacio de la Magdalena, Santander, Spain June 20–24
- Theme-"Earth Analog Environments and the Search for Life Beyond the Earth"
- Interdisciplinary examination of the chemical, physical, and geological properties of potential extraterrestrial habitats and an in-depth description and analysis of sites on Earth with similar characteristics. In particular, lectures and activities considered icy satellites, rocky planets in the Solar System, extreme Earth environments, and terrestrial exoplanets.
- I evaluated talks and posters for the biological sciences section of the undergraduate forum March 30, 2016. Prior to this, judges attended a two-hour training session. Applicants were rated on (1) poster presentation (if applicable) :including research content, visual graphics, grammar, and conclusions; (2) oral presentation including appropriate terminology, poise/presentation, knowledge of work, and organization/flow.
- The Ohio State University Viromics training course, Columbus, OH
- The course was held Feb. 8-9 and training included the iVirus pipeline part of the CyVerse infrastructure and MetaVir. With iVirus we learned how to quality check metagenomics sequences, assembly, VirSorter, and mapping the reads to contigs for coverage. MetaVir is a web server designed to annotate viral metagenomic sequences. With these tools, we were able to give our data ecological meaning.
- Directors: Drs. Matthew Sullivan, Simon Roux, and Ben Bolduc
- Sky School is 1-5 day Science programs for Arizona K-12 students at the 25-acre Mt. Lemmon campus. My fellowship was for the 2014-2015 school year. Programs focus on core UA science areas :such as sky island ecology, biology, geology, and astronomy and have been developed in collaboration with local school districts to meet Arizona State and Next Generation Science :Standards. I specifically bridge biology and astronomy and share the field of astrobiology with the students. Fellowship Responsibilities include participating in at least 4-5 Sky School :events in during each semester, in which leading daytime activities and participating in evening activities. Daytime activities are for a group of 5-10 students and include sample :collection, learning projects, and demonstrations. 3+ day trips include a science inquiry project, which is presented before they leave, and an in-depth learning experience on the :graduate fellows field of study.
- Director Dr. Alan Strauss
- March 14-15, 2015 I was a co-exhibitor for the Sky School booth at Science City part of the Tucson Festival of Books. It is the 2nd largest book festival and largest book and science :festival. Our booth had several meteorite samples and thin sections that students could view under the microscopes. We also had some neat giveaways to encourage and promote STEM :education. We also had material on the OSIRIS-Rex mission led by NASA. This huge festival draws a diverse group of people from all over the world. This year we got a special visit from :former Arizona congresswoman Gabrielle Giffords.
- On Feb. 23, 2015 from 7:30-3:30 I was a co-exhibitor for the Sky School both at Sahuarita middle school SciTech festival. There were 480 6th-8th graders that participated and went to each :booth to answer questions in their “passport”. The Shy School booth question was: How can meteorites give us information on the formation of the solar system. Our booth had several :meteorite samples and thin sections that students could view under the microscopes. We also had material on the OSIRIS-Rex mission led by NASA.
- Contact: Rebecca Lipson (email@example.com)
- October 4 and 18, 2014, I led an Earth Sciences outreach event for visitors of B2 from 4-8pm. Each event incorporated geology and microbiology and was catered to that date’s theme.
- Contact: Dr. Pacifica Sommers (firstname.lastname@example.org)
- I reviewed and evaluated travel grant applications submitted to UA GPSC for the Nov. 1, 2014 deadline. Applicants were rated on four topics: (1) professional development, (2) description :of their work, (3) description of the event and the student’s efforts to secure funding, and (4) their proposed budget for the event.
- On September 5, 2014 I led an Earth Sciences day outreach event for all the 3rd-grade classes (44 students) at Lake Pleasant Elementary. We taught each object of strand 6: Earth and Space :Science from the Arizona Science Standards. The day started with learning about the layers of the Earth, then minerals, the three rock types, the rock cycle, fossils, and fossil fuel. :Songs, activities, and demonstrations were incorporated, including my famous Cheetos fossil experiment.
- Contact: Mrs. Michelle Kist (email@example.com)
- A 5-week intense course. The first two weeks involved traveling around Southern California and Nevada collecting stromatolites, environmental, and microbial samples. Sample sites included :Walker Lake, Mt. Dunfee, Rowlands Reef, and Little Hot Creek. Weeks 3 and 4 were at CSU-Fullerton processing samples and coming up to speed on modern geological and biological techniques. :The final week was at the USC Wrigley institute for environmental studies on Catalina Island, where we pulled data and analyses to write and present a final report.
- Dates: June 8-July 10, 2014
- Final project and presentation: How do microbial mats become stromatolites?
- Directors: Dr. Frank Corsetti (firstname.lastname@example.org) and Dr. John Spear (email@example.com)
- Earth Week & SWESX coordinator, UA, Tucson, Arizona
- School of Earth & Environmental Sciences (SEES) hosted EarthWeek at UA. The SWES department had their own designated day full of oral presentations by undergraduate and graduate students. :In 2014, I was in charge of student recruitment for presentations for the SWES department. I reviewed oral & poster abstracts that were submitted, ordered food, created sessions, reserved :rooms, and judged posters. For 2015 I was chair of the SWES department and a member of the UA SEES EarthWeek. I continued my previous year's duties, along with acting as social chair for :the University; this included planning several trivia nights to encourage city-wide participation in EarthWeek.
- La Cima Middle School Career Shadow Day, Tucson, Arizona
- I hosted four students for two hours interested in marine microbiology. For the first hour, I led a discussion about aspects of the field, necessary education, and career options. The :second hour involved a tour of the laboratory involving a discussion of equipment and methods.
- La Cima coordinator: Vaughn Croft (firstname.lastname@example.org)
- Saturday Science Academy coordinator, University of Arizona
- As the event coordinator I confirmed logistics for the event with Arizona MESA (a university-based outreach program). I planned the activity schedule, recruited activity leaders, :identified keynote speakers, arranged for activity supplies and student prizes, organized volunteers, taught activities to volunteers, ordered snacks for students, provided feedback to :volunteers, and led the event. The goal was to excite ~80 underrepresented middle school students about STEM fields, college, and sponsor future science-related events. In February 2014 :the theme was "Astonishing Astrobiology" and in November 2014, the theme was "A Geological Perspective on Life".
- DRI representative, Marsfest 2013 in Death Valley National Park
- March 1-3, 2013 a festival celebrating extreme environments was held at Death Valley National Park to get the public involved in STEM activities. The agenda included presentations, booth :expositions, and field trips (Badwater basin and other park sites). I presented posters to the public, showcased DRI, and collected samples on field trips with youth for viewing under a :microscope. Attendees also camped at the park overnight and participated in astrological activities.
- Graduate outreach aid, University of Nevada, Reno for George L. Dilworth Middle School
- We visited weekly through the fall and episodically through the spring. Topics and activities included microbiology mysteries (e.g., 1854 cholera outbreak, 1993 Milwaukee Cryptosporidium :outbreak), chemistry demonstrations (e.g., vinegar and baking soda, Coke and Mentos), and math lessons (e.g., calculating how much water we use in a year). Primary activities included :interacting with students to solve science problems, working with students to develop STEM research projects, and participating in career day.
- International Stable Isotope Biogeochemistry summer course at Michigan State University (Summer :2012)An intensive one week course. Mornings were filled with lectures on the different types of Mass spectroscopy, carbon, nitrogen, and sulfur cycling, and the stable isotopes of C, H, O, S, and N. Afternoon and evenings consisted of lab rotations analyzing and interpreting samples.
- Dates: May 7-11 2012
- Instructors: Dr. Nathaniel Ostrom (email@example.com) and
- Dr. Peggy Ostrom (firstname.lastname@example.org)
- Preceptor, University of Arizona, NATS 101: A Geological Perspective
- Class was three units with a mandatory discussion session. I co-taught the discussion for a semester with a graduate student; taught the discussion session independently for next three :semesters (25-30 students). The focus was on a single geological topic with a group assignment. I graded all the assignments and submitted the scores. Professor: :Dr. Jessica Kapp
- National Society of Collegiate Scholars
- National Scholars Honor Society
- Phi Kappa Phi honor society
- Golden Key International Honour Society
- National Blue Key Honorary (UA, 2009-2011)
- Hope L. Jones Scholarship (2008)
- W.H. Fuller Scholarship (2009)
- T. F. Buehrer Scholarship (2010)
- George L. Jones Scholarship (2010)
- Great Lakes National Scholarship Program recipient (2013, $2,500)
- UNR Graduate Student Association (GSA) travel grants (Fall 2011 for AGU, $500; Spring 2012 ASM, $500; Fall 2012 for AGU, $500)
- UNR GSA’s poster competition 3rd place (2013, $650)
- DRI poster competition 1st place (2013, $200)
- Graduate student representative for the Division of Earth and Ecosystems at DRI (Fall 2011- Spring 2013)
- College of Agriculture, Biotechnology, and Natural Resource’s Outstanding Graduate Student 2013
- SWES graduate student representative (Fall 2013- Spring 2015)
- Student Representative for UA Global Change minor committee (Fall 2014- Spring 2015)
- SWES 2013-2014 excellent graduate student ($5,000)
- SWES 2014-2015 excellent graduate student ($1,000)
- H. E. Carter Travel Award ($600; 2014)
- GPSC Travel Award ($750; 2014)
- SWES Travel Award ($300; 2014)
- Sky School fellowship ($4,000; 2014–2015)
- United States Permafrost Association Travel Award ($500; 2014)
- Great Lakes National Scholarship Program recipient (2015, $2,500)
- Full scholarship to the 2016 Nasa astrobiology institute summer school in Santander, Spain (2016, ~$3,000)
- Great Lakes National Scholarship Program recipient (2016, $2,500)
- Travel award to attend Astrobiology Science Conference 2017 (AbSciCon, $500)
- DOE-SCGSR fellowship ($18,000; June 4–November 1, 2018)
- Best poster award at LLNL postdoc symposium (May 1, 2019)