User:Fermenter User/Notebook/DOE Workshop 20110405

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WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When: April 5th - April 14th 2011

Where: LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Ann Y.K. Wong: Assistant Instructor, Ph.D. previously MacGillivray lab
  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati N. Yewalkar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Shiau-Yun (Mary) Tang (UBC Alumni)


Group A:

(After you log in with your personal password, click the "EDIT" button on the right side of GROUP A and list your name below)

Chris Tam
Louise Creagh

Group B:

(After you log in with your personal password, click the "EDIT" button on the right side of GROUP B and list your name below)

MARY TANG
Swati Yewalkar






Day -1 (April 4th, Mon)

Runs ' ' 1, 2, 3
07:30
08:00
09:00
09:30 SH: Assemble bioreactors
09:50 SH: Autoclve
10:00
10:30
11:10 SH: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS

Runs ' 1, 2, 3 4, 5, 6
pictures
run 1, 2, 3 (left to right)
08:30 SH: Sampling
09:30 Ann: Prepare high density K91 cells Group A: Assemble bioreactors
09:50 Group A: Autoclave
10:40 Presentation Presentation
12:00 Ann: Phage infection Group A: Install bioreactors
13:00 Ann: Spot titer
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # Introduction about our Facility and Services
 # Introduction about bioreactors
 # How to assemble bioreactors


Power Outage started around 8pm and lasted approximately 2.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #4 losted power during fermentation. End point looked ok. 
Run #5 was not affected by power. However, pH control was not done properly. 
Run #6 was not affected by power. End point looked ok.
=> Run #5 will be repeated at last as additional runs.

Day 2 (April 6th, Wed)

Runs 1, 2, 3 4, 5, 6 7, 8, 9
pictures
run 1,2,3 (left to right)
run 4,5,6 (left to right)
08:00 Ann: Colony counting SH: Sampling
09:00 Ann: Prepare high density K91 cells Group B: Assemble bioreactors
09:50 Group B: Autoclave
10:00 Presentation Presentation Presentation
11:00 Ann: Phage infection Group B: Install bioreactors
12:00 Ann: Spot titer
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K91 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # Fermentation Database and Websites
 # How to Install Bioreactors
 # How to Setup Bio-controllers

Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #7 losted power during fermentation. End point looked ok. 
Run #8 was not affected by power. End point looked ok. 
Run #9 was not affected by power. End point looked ok.
=> Run #7 will be repeated at last as additional runs.

Day 3 (April 7th, Thu)

Runs 4, 5, 6 7, 8, 9 10, 11, 12
pictures
run 4,5,6 (left to right)
run 7,8,9 (left to right)
08:00 Ann: Colony counting SH: Sampling
09:00 Ann: Prepare high density K91 cells
<--didn't grow very well due to power outage
Group A: Assemble bioreactors
09:50 Group A: Autoclave
10:00 Presentation Presentation Presentation
11:00 Group A: Install bioreactors
12:00
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K91 cells
17:00 SH: Inoculation


10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 1. Design Experiments

Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite.
One was connected to a computer and one was connected to incubator.
Also, each fermenter is now connected to batteries to provide over 500 min capacity.

Day 4 (April 8th, Fri)

Runs 7, 8, 9 10, 11, 12 11, 13, 14
pictures
colony 7,8,9 (left to right)
run 10,11,12 (left to right)
08:00 SH: Sampling
09:00 Ann: Prepare high density K91 cells SH: Assemble bioreactors
09:50 SH: Autoclave
11:00 Ann: Phage Infection SH: Install bioreactors
12:00 Ann: Spot titering
15:30 Thaw stock
16:50
17:00 Inoculation




NO PRESENTATION

Day 5 (April 9th, Sat)


There is a scheduled power outage on Saturday morning between 8:00 AM and 10:00 AM
=> Power outage began at 8:01 and resumed at 10:00.
No problem occured during this power outage

Runs 6, 8, 9, 10, 12 11, 13, 14 '
pictures
run 10, 12, (left to right)
run 11,13,14 (left to right)
08:00 SH: Colony counting SH: Sampling


NO PRESENTATION

Day 6 (April 10th, Mon)

Runs ' ' 15, 16, 17
07:30
08:00
09:00
09:30 Group B: Assemble bioreactors
09:50 Group B: Autoclve
10:00
10:30
11:10 Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection

Day 7 (April 11th, Tue)

Runs 11, 13, 14 15, 16, 17 18, 19, 20
pictures
run 11, 13, 14 (left to right)
run 15,16,17 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 SH: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics

Day 8 (April 12th, Wed)

Runs 15, 16, 17 18, 19, 20 4, 5, 7
pictures
run 15, 16, 17 (left to right)
run 18,19,20 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check SH: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis: Model Graph and Step 4. Optimization

Day 9 (April 13th, Thu)

Runs 18, 19, 20 4, 5, 7 '
pictures
run 18, 19, 20 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check SH: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00
10:00 - 11:00 Presentation (Rm 4.303)
 # Case Studies: Publication

Day 10 (April 14th, Fri)

Runs 4, 5, 7 ' '
07:30
08:00
09:00 Group B: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!






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