WORK B1
1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop
Teaching:
Where:
LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332
Attending:
Ann Y.K. Wong : Assistant Instructor, Ph.D. previously MacGillivray labLouise Creagh : Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular BiophysicsRaymond Pan : PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)Swati N. Yewalkar : Postdoctoral Fellow, Department of Chemical and Biological EngineeringShiau-Yun (Mary) Tang (UBC Alumni)
Group A:
(After you log in with your personal password, click the "EDIT" button on the right side of GROUP A and list your name below)
Chris Tam
Group B:
(After you log in with your personal password, click the "EDIT" button on the right side of GROUP B and list your name below)
MARY TANG
Day -1 (April 4th, Mon)
Runs
'
'
1, 2, 3
07:30
08:00
09:00
09:30
SH: Assemble bioreactors
09:50
SH: Autoclve
10:00
10:30
11:10
SH: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
Day 1 (April 5th, Tue) <-- WORKSHOP STARTS
Runs
'
1, 2, 3
4, 5, 6
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 1, 2, 3 (left to right)
08:30
SH: Sampling
09:30
Ann: Prepare high density K91 cells Group A: Assemble bioreactors
09:50
Group A: Autoclave
10:40
Presentation
Presentation
12:00
Ann: Phage infection Group A: Install bioreactors
13:00
Ann: Spot titer
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# Introduction about our Facility and Services
# Introduction about bioreactors
# How to assemble bioreactors
Power Outage started around 8pm and lasted approximately 2.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #4 losted power during fermentation. End point looked ok.
Run #5 was not affected by power. However, pH control was not done properly.
Run #6 was not affected by power. End point looked ok.
=> Run #5 will be repeated at last as additional runs.
Day 2 (April 6th, Wed)
Runs
1, 2, 3
4, 5, 6
7, 8, 9
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 1,2,3 (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 4,5,6 (left to right)
08:00
Ann: Colony counting SH: Sampling
09:00
Ann: Prepare high density K91 cells Group B: Assemble bioreactors
09:50
Group B: Autoclave
10:00
Presentation
Presentation
Presentation
11:00
Ann: Phage infection Group B: Install bioreactors
12:00
Ann: Spot titer
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K91 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# Fermentation Database and Websites
# How to Install Bioreactors
# How to Setup Bio-controllers
Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #7 losted power during fermentation. End point looked ok.
Run #8 was not affected by power. End point looked ok.
Run #9 was not affected by power. End point looked ok.
=> Run #7 will be repeated at last as additional runs.
Day 3 (April 7th, Thu)
Runs
4, 5, 6
7, 8, 9
10, 11, 12
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 4,5,6 (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 7,8,9 (left to right)
08:00
Ann: Colony counting SH: Sampling
09:00
Ann: Prepare high density K91 cells <--didn't grow very well due to power outage Group A: Assemble bioreactors
09:50
Group A: Autoclave
10:00
Presentation
Presentation
Presentation
11:00
Group A: Install bioreactors
12:00
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K91 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 1. Design Experiments
Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite.
One was connected to a computer and one was connected to incubator.
Also, each fermenter is now connected to batteries to provide over 500 min capacity.
Day 4 (April 8th, Fri)
Runs
7, 8, 9
10, 11, 12
11, 13, 14
pictures
Error creating thumbnail: Unable to save thumbnail to destination colony 7,8,9 (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 10,11,12 (left to right)
08:00
SH: Sampling
09:00
Ann: Prepare high density K91 cells SH: Assemble bioreactors
09:50
SH: Autoclave
11:00
Ann: Phage Infection SH: Install bioreactors
12:00
Ann: Spot titering
15:30
Thaw stock
16:50
17:00
Inoculation
NO PRESENTATION
Day 5 (April 9th, Sat)
There is a scheduled power outage on Saturday morning between 8:00 AM and 10:00 AM
=> Power outage began at 8:01 and resumed at 10:00.
No problem occured during this power outage
Runs
6, 8, 9, 10, 12
11, 13, 14
'
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 10, 12, (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 11,13,14 (left to right)
08:00
SH: Colony counting SH: Sampling
NO PRESENTATION
Day 6 (April 10th, Mon)
Runs
'
'
15, 16, 17
07:30
08:00
09:00
09:30
Group B: Assemble bioreactors
09:50
Group B: Autoclve
10:00
10:30
11:10
Group B: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection
Day 7 (April 11th, Tue)
Runs
11, 13, 14
15, 16, 17
18, 19, 20
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 11, 13, 14 (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 15,16,17 (left to right)
07:30
SH: Prepare hight density K19 cells
08:00
SH: Prepare dilution tubes
09:00
SH: Sampling
09:30
Ann: Phage dilution Group A: Assemble bioreactors
09:50
Ann: OD of HD cells Group A: Autoclve
10:00
Ann: Dry plates
10:30
Ann: Infect K19 cells
11:10
Ann: Spot titer Group A: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics
Day 8 (April 12th, Wed)
Runs
15, 16, 17
18, 19, 20
4, 5, 7
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 15, 16, 17 (left to right) Error creating thumbnail: Unable to save thumbnail to destination run 18,19,20 (left to right)
07:30
SH: Prepare hight density K19 cells
08:00
SH: Prepare dilution tubes
09:00
Ann: Colony check SH: Sampling
09:30
Ann: Phage dilution Group B: Assemble bioreactors
09:50
Ann: OD of HD cells Group B: Autoclve
10:00
Ann: Dry plates
10:30
Ann: Infect K19 cells
11:10
Ann: Spot titer Group B: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis: Model Graph and Step 4. Optimization
Day 9 (April 13th, Thu)
Runs
18, 19, 20
4, 5, 7
'
pictures
Error creating thumbnail: Unable to save thumbnail to destination run 18, 19, 20 (left to right)
07:30
SH: Prepare hight density K19 cells
08:00
SH: Prepare dilution tubes
09:00
Group A: Colony check SH: Sampling
09:30
Ann: Phage dilution
09:50
Ann: OD of HD cells
10:00
Ann: Dry plates
10:30
Ann: Infect K19 cells
11:10
Ann: Spot titer
15:30
16:50
17:00
10:00 - 11:00 Presentation (Rm 4.303)
# Case Studies: Publication
Day 10 (April 14th, Fri)
Runs
4, 5, 7
'
'
07:30
08:00
09:00
Group B: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)
Helful links http://meta.wikimedia.org/wiki/Table http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!
Recently Edited Notebook Pages 
