User:Ferdinando Pucci/Notebook/TEMs detection improvement/2008/05/07
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Transfer plasmids previously produced:
Each vector produced in a 10-cm plate. For Tie2 vectors I used 18ug of transfer plasmid, 15ug for tetO7 (smaller). I used 5ug for both packaging plasmids:
Only for CCL systems, added 1mM Na-butyrate in the medium (7ml) 12-14h after transfection.