User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/16

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Experimental Biological Chemistry Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


To synthesize nanoparticles and investigate the effects of changing the concentration of the reactants, while keeping the molar ratio constant, and to determine what solvents can be used to dissolve the purple fibers.


Nanoparticle Synthesis

Four reactions were performed. Three epitube reactions and one large glass test tube reactions were set up. The large test tube reaction consisted of 1 mL of 30uM BSA, 0.5 mL of 4.17 mM HAuCl4, and 8.5 mL water. The tube was placed in an oven set to 70°C for 3 hours.

Epitube 1 - 100 uL BSA, 50 uL HAuCl4, and 850 uL water. Final Concentrations:

Epitube 2 - 25 uL HAuCl4, 50 uL BSA, and 925 uL water in this order. Final Concentrations:

Epitube 3 - 50 uL BSA, 25 uL HAuCl4, and 925 uL water in this order. Final Concentrations:

Dissolving Purple Fibers The fibers were dissolved in four different solutions.

Solution 1 - 1 mL of 1 M Tris

Solution 2 - 1 mL of 6 M NaOH

Solution 3 - 1 mL of DMSO

Solution 4 - 1 mL of DMSO

A small clump of fibers was added to solutions 1, 2, and 3. Due to the success of dissolving fibers in solution 3, a large amount of fibers was added to solution 4.


  • The large reaction tube had a dark vibrant purple color.
  • Epitubes 2 and 3 had the same color: a light pink-purple. Epitube 1 had a darker color than tubes 2 and 3. It was a light-medium purple color.
  • Fibers did not dissolve in solution 1. After 1 week, the fibers dissolved in solution 2. All of the fibers dissolved instantly in solution 2. A large amount of the fibers dissolved instantly in solution 4. After 1 week, the majority of the fibers had dissolved and the solution became a dark purple color. A small amount of tiny fibers could be seen floating around.


This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.