User:Eleni N. Kalivas/Notebook/CHEM-571/2014/10/01

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Objective Make stock concentrations (one set for both groups) Take UV-Vis and Fluorescence spectra for each stock Make a calibration curve for both fluorescence and UV-Vis Procedure Preparation of stock solutions 0.0120g of R6G in 10mL needed to make the initial stock solution of 2500uM, this was used to prepare a secondary stock of 500uM, used to make the dilutions for 0.1, 0.5, 0.75, 1.0, 1.2 and 1.5. The initial stock of 2500uM was used to make a 2uM. But the 1.5 and 2 uM were not used for the calibration curve because the fluorescence was too high that it did not provide accurate data. table of stock solution preparation Concentration of stock solution (M) Amount of Stock used (mL) 0.1 0.001 0.5 0.005 0.75 0.0075 1 0.01 1.2 0.012 Fluorescence Parameters of Fluorimeter: 500nm excitation 515-700nm scan range 10.0nm slit width 200nm/min scan speed Fluorescence spectra of standards Calibration curve based on Fluorescence Fluorescence of PVA/PVAC films and glass absorbance standards UV-Vis UV-Vis spectra of standards Calibration curve based on UV-Vis UV-Vis of PVA/PVAC films and glass absorbance standards