User:Eleni N. Kalivas/Notebook/CHEM-571/2014/10/01

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Objective

  1. Make stock concentrations (one set for both groups)
  2. Take UV-Vis and Fluorescence spectra for each stock
  3. Make a calibration curve for both fluorescence and UV-Vis

Procedure

Preparation of stock solutions

0.0120g of R6G in 10mL needed to make the initial stock solution of 2500uM, this was used to prepare a secondary stock of 500uM, used to make the dilutions for 0.1, 0.5, 0.75, 1.0, 1.2 and 1.5. The initial stock of 2500uM was used to make a 2uM. But the 1.5 and 2 uM were not used for the calibration curve because the fluorescence was too high that it did not provide accurate data.

  • table of stock solution preparation
Concentration of stock solution (M) Amount of Stock used (mL)
0.1 0.001
0.5 0.005
0.75 0.0075
1 0.01
1.2 0.012

Fluorescence

  • Parameters of Fluorimeter:
    • 500nm excitation
    • 515-700nm scan range
    • 10.0nm slit width
    • 200nm/min scan speed


  • Fluorescence spectra of standards

2014 1001 R6G fluor.PNG

  • Calibration curve based on Fluorescence

Screen Shot 2014-10-03 at 2.38.20 PM.png

  • Fluorescence of PVA/PVAC films and glass absorbance standards

Screen Shot 2014-10-03 at 2.02.41 PM.png

UV-Vis

  • UV-Vis spectra of standards

2014 1001 R6G abs.PNG

  • Calibration curve based on UV-Vis

2014 1001 R6G calibration.PNG

  • UV-Vis of PVA/PVAC films and glass absorbance standards