User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/04

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Objective

Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.

Protocol

  1. Remove 1 mL aliquot from test tube and place in the cuvette.
  2. Take the UV-Vis spectra of the sample.
  3. Take the fluorescence spectra of each sample
  4. Repeat for each test tube
  5. Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
  6. Data analysis of the UV-Vis absorbances, and fluorescence spectra
  1. Determine if any changes in procedure need to be made.
    1. Is a different scale of protein:ligand ratio required?
    2. Were QD NCs successfully synthesized?
  2. Prepare stock solutions for next synthesis on the following day.

Note: Much of the protocol for today will be determined by the results of our first synthesis.

Edited Protocol

  • we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.


Corrected Volumes Table

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM L-Cysteine (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
0 540 60 600 0 600 3000 600 5.4
10 540 60 600 60 540 3000 600 5.4
20 540 60 600 120 480 3000 600 5.4
30 540 60 600 180 420 3000 600 5.4
40 540 60 600 240 360 3000 600 5.4
50 540 60 600 300 300 3000 600 5.4
60 540 60 600 360 240 3000 600 5.4
70 540 60 600 420 180 3000 600 5.4
80 540 60 600 480 120 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

Notes

Add before and after photos, and get uv-light photo.
UV-Vis of BSA-L-Cysteine Bsa-cys.png