User:Edward M. Perkins

Contact Info

Edward M. Perkins (an artistic interpretation)

• Edward M. Perkins (Ned)
• Johns Hopkins University
• 3400 North Charles St.
• Room 104 Dunning Hall.
• Baltimore, MD, 21218
• Email me through OpenWetWare

I work in the Integrated Imaging Center at Johns Hopkins University. I learned about OpenWetWare from Federick Tan in Blake Hill's group at JHU, and I've joined because I would like to try using a wiki for basic documentation of our imaging center's facilities. Hopefully it will encourage users to share tips, tricks and refinements of protocols.

Education

• MS candidate, Johns Hopkins University
• 2004, BA, Computer Science, Skidmore College

Research interests

1. Single Particle Reconstruction
2. Fluorescence Correlation Spectroscopy
3. Immuno electron microscopy
4. Pinhole photography

Publications

1. Daniels BR, Perkins EM, Dobrowsky TM, Sun SX, and Wirtz D. Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion. J Cell Biol. 2009 Feb 23;184(4):473-9. DOI:10.1083/jcb.200809077 |
2. Henson BW, Perkins EM, Cothran JE, and Desai P. Self-assembly of Epstein-Barr virus capsids. J Virol. 2009 Apr;83(8):3877-90. DOI:10.1128/JVI.01733-08 |
3. Perkins EM, Anacker D, Davis A, Sankar V, Ambinder RF, and Desai P. Small capsid protein pORF65 is essential for assembly of Kaposi's sarcoma-associated herpesvirus capsids. J Virol. 2008 Jul;82(14):7201-11. DOI:10.1128/JVI.00423-08 |
4. Maldonado-Báez L, Dores MR, Perkins EM, Drivas TG, Hicke L, and Wendland B. Interaction between Epsin/Yap180 adaptors and the scaffolds Ede1/Pan1 is required for endocytosis. Mol Biol Cell. 2008 Jul;19(7):2936-48. DOI:10.1091/mbc.e07-10-1019 |
5. Huang E, Perkins EM, and Desai P. Structural features of the scaffold interaction domain at the N terminus of the major capsid protein (VP5) of herpes simplex virus type 1. J Virol. 2007 Sep;81(17):9396-407. DOI:10.1128/JVI.00986-07 |
6. Perkins EM and McCaffery JM. Conventional and immunoelectron microscopy of mitochondria. Methods Mol Biol. 2007;372:467-83. DOI:10.1007/978-1-59745-365-3_33 |
7. Solé M, Perkins EM, Frisancho A, Huang E, and Desai P. The N terminus of the herpes simplex virus type 1 triplex protein, VP19C, cannot be detected on the surface of the capsid shell by using an antibody (hemagglutinin) epitope tag. J Virol. 2007 Aug;81(15):8367-70. DOI:10.1128/JVI.00819-07 |
8. Ingerman E, Perkins EM, Marino M, Mears JA, McCaffery JM, Hinshaw JE, and Nunnari J. Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol. 2005 Sep 26;170(7):1021-7. DOI:10.1083/jcb.200506078 |

Useful links

JHU Integrated Imaging Center

• Introductory tutorial
• OpenWetWare help pages