User:Douglas P Shepherd

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Contact Info

Douglas P Shepherd
  • Douglas P Shepherd
  • Assistant Professor
  • Department of Physics
  • Campus Box 157
  • University of Colorado Denver
  • Denver, CO 80217-3364
  • Google Scholar
  • Email me

Research and Teaching interests

My research interests are currently focused on understanding how the variability in genetic expression among single cells in a population determines the overall phenotype of the population. We work with a variety of single-molecule spectroscopic, biological labeling, and computational techniques to achieve this goal.

My teaching interests are broadly focused on helping students develop communication, problem-solving, and critical thinking skills to apply throughout their life. I actively foster communication and collaboration between the various scientific disciplines (e.g. biology, physics, mathematics) at an earlier stage than graduate school by teaching cross-listed Biophysics classes and providing a multidisciplinary research laboratory that engages both undergraduate and graduate students.


  • 2011, PhD Physics, Colorado State University
  • 2003, BS Physics, University of California Santa Barbara


  1. Enhanced 3D localization of individual RNA transcripts via astigmatic imaging, Evan P. Perillo, Leyma De Haro, Jennifer S. Martinez, Hsin-Chih Yeh, Andrew K. Dunn, Douglas P. Shepherd, James H. Werner, Proc. of SPIE, accepted 2014
  2. Using mixture tuned match filtering to measure changes in subpixel vegetation area in Las Vegas, Nevada, Christa M Brelsford, Douglas P Shepherd, Journal of Applied Remote Sensing, accepted 2014
  3. Counting Small RNA in Pathogenic Bacteria, Douglas P. Shepherd, Nan Li, Sofiya N. Micheva-Viteva, Brian E. Munsky, Elizabeth Hong-Geller, James H. Werner, Analytical Chemistry 85 (10), 4938-4943 2013
  4. In Situ Studies of Photoluminescence Quenching and Photocurrent Yield in Quantum Dot Sensitized Single Crystal TiO2 and ZnO Electrodes, Douglas P. Shepherd, Justin B. Sambur, Yong-Qi Liang, Bruce A. Parkinson, Alan K. Van Orden, Journal of Physical Chemistry C, 116 (39), 421069-21076 2012
  5. New tools for discovering the role sRNA plays in cellular regulation, Douglas P. Shepherd, Nan Li, Sofiya N. Micheva-Viteva, Brian E. Munsky, Elizabeth Hong-Geller, James H. Werner Proc. of SPIE Vol 8228, 822808-1 2012
  6. Fluorescence intermittency and energy transfer in small clusters of semiconductor quantum dots, Douglas P. Shepherd, Kevin J. Whitcomb, Kenneth K. Milligan, Peter M. Goodwin, Alan K. Van Orden Journal of Physical Chemistry C, 114 (35), 14831-14837 2010