Tasklist
- Finish what you started on Wednesday
- Attach rubber hose to end of a plastic column
- Weigh the empty, dried column
- Rinse with water, allow water to drain through column, dry outside, and reweigh
- Add enough beads to fill the column 1/3 - 1/2
- Cap and tilt back and forth until no air bubble present
- Rinse with water, allow water to drain through column, dry outside, and reweigh
- Dilute 10 mL of MG stock to 50 ppm
- Pour the MG through your column, 5 - 10 mL at a time
- Collect 5 mL of affluent at a time
- Rinse with 10 mL of HPLC water, collecting in 2 vials
- What do you think the void volume of your column is? Why does it matter? How can you measure it?
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