User:Douglas M. Fox/Notebook/AU CHEM-581 F2012 Lab Support/2016/09/16

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Tasklist Notebooks!!! Finish what you started on Wednesday Attach rubber hose to end of a plastic column Weigh the empty, dried column Rinse with water, allow water to drain through column, dry outside, and reweigh Add enough beads to fill the column 1/3 - 1/2 Cap and tilt back and forth until no air bubble present Rinse with water, allow water to drain through column, dry outside, and reweigh Dilute 10 mL of MG stock to 50 ppm Pour the MG through your column, 5 - 10 mL at a time Collect 5 mL of affluent at a time Rinse with 10 mL of HPLC water, collecting in 2 vials What do you think the void volume of your column is? Why does it matter? How can you measure it?