User:Dominique Brager/Notebook/481 Notebook/2017/10/17

Error creating thumbnail: Unable to save thumbnail to destination Biomaterials Design Lab

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Objective Prepare reducing agent stock solutions 5 - 10 mL of 100 mM each fructose, galactose, sucrose, Na3Ct, H2Asc For chitosan (168 g/mol), prepare 10 mL 50 mM. Add 4.75 mL HCl per g of chitosan or 0.265 mL HAc per g of chitosan For cellulose nanocrystals, use a 4% solution AuNP for 100 mM stock solutions 0.1 mL 10 mM HAuCl4 + 0.1 mL 100 mM reducing agent + 3.8 mL H2O For cellulose, add 0.1 mL 10 mM HAuCl4 to 3.9 mL cellulose 0.2 mL 10 mM HAuCl4 + 3.8 mL NaBH4 Place test tubes in water bath (600 mL beaker) at 80 C until color changes (~30 min) Measure UV-VIS of your solutions Data Error creating thumbnail: Unable to save thumbnail to destination Au-His-Na has a peak at 526. Au-His-NH4 has an even higher peak at 537. (Different peak wavelengths indicate different sizes of NP). Au-Cys-NH4 has a sharp peak at 370. Error creating thumbnail: Unable to save thumbnail to destination The first three solutions (both Histidines and the Cys with Na) didn't have any peaks. The last one Ag Cys NH4 has a shoulder at around 300 indicating that there was some nanoparticle formation. Error creating thumbnail: Unable to save thumbnail to destination Zn-His-Na formed a white solid in solution. UV spectrum maxes out due to solid interference. Zn-His-NH4 does not have a peak. Zn-Cys-Na has a peak at 263. Zn-Cys-NH4 has a peak at 282. Error creating thumbnail: Unable to save thumbnail to destination AuZn-His-Na has a peak at 280. AuZn-His-NH4 has a peak at 263. Error creating thumbnail: Unable to save thumbnail to destination We measured the solutions that we thought may have coloring. The ascorbic acid NP had color before we put them in the bath but by the time we measured them, there were no peaks. The Na2Ct did have peaks though: One around 260 and the other at 527. Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.