User:Dominique Brager/Notebook/481 Notebook/2016/10/19

Error creating thumbnail: Unable to save thumbnail to destination Biomaterials Design Lab

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Objective Prepare heavy metal solutions (Zn, Pb, Cu) Description Prepare 100mL of 100ppm solutions of Pb, Zn, Cu Cu 100mL*1L/1000mL*100mg Cu/1L*134.45 g/mol CuCl2/63.54 g/mol Cu = 21.16 mg CuCl2 Pb 100mL*1L/1000mL*100mg Pb/1L*325.29 g/mol PbAc/207.2 g/mol Cu = 15.7 mg PbAc Zn 100mL*1L/1000mL*100mg Zn/1L*138.315 g/mol ZnCl2/65.38 g/mol Zn = 21.15 mg ZnCl2 Prepare 50mL of 300 ppm metals (100ppm Pb, 100ppm Zn, 100ppm Cu) 10.58 mg CuCl2 10.57 mg ZnCl2 7.85 mg PbAc 50mL H2O Dilution to 60ppm 10mL of 300ppm and 40mL water 20% column data: Dry:15.5904g Wet: 15.9570g W/Beads: 16.65g 10% column data Dry:15.966g Wet: 16.389g W/Beads: 17.55g Ran data analysis on 10% beads with MG Found the Max Absorbance for each run. Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample. Created plot of concentration vs. Volume Eluted. Calculated the amount of MG added to the Column 54 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg Calculated the amount of MG eluted. From samples 1-54: 5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed For sample 55: 1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed 4mL*1L/1000mL*concentration of sample = mg of MG desorbed For samples 56-88: 5mL*1L/1000mL*concentration of sample = mg of MG desorbed Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column. Find capacity of beads by dividing mass of MG adsorbed by mass of beads Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads. Data Data and Results for 10% Column with MG Graph of Concentration of MG vs. Volume of MG Eluted Error creating thumbnail: Unable to save thumbnail to destination Amount of MG loaded: 13.5mg Amount not adsorbed: 0.6025mg Amount adsorbed: 13.5mg-0.6025mg=12.9mg Amount desorbed: 0.2879mg Mass of Wet Beads in Column: 1.465g Mass of Dry Beads in Column: 0.2683g Capacity of Beads: 48.08 mg/g Mass of Clay in Column: 0.02683g Capacity of Clay: 480.8 mg/g Swelling for 10% clay is 446% 4.46=wet-dry/dry 4.46*dry = wet-dry 4.46dry+dry= wet 5.46dry = wet wet = 1.465g 1.465/5.46 = dry dry mass = 0.2683g