# User:Dominique Brager/Notebook/481 Notebook/2016/10/19

Biomaterials Design Lab Main project page
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## Objective

• Prepare heavy metal solutions (Zn, Pb, Cu)

## Description

• Prepare 100mL of 100ppm solutions of Pb, Zn, Cu
• Cu
100mL*1L/1000mL*100mg Cu/1L*134.45 g/mol CuCl2/63.54 g/mol Cu = 21.16 mg CuCl2
• Pb
100mL*1L/1000mL*100mg Pb/1L*325.29 g/mol PbAc/207.2 g/mol Cu = 15.7 mg PbAc
• Zn
100mL*1L/1000mL*100mg Zn/1L*138.315 g/mol ZnCl2/65.38 g/mol Zn = 21.15 mg ZnCl2
• Prepare 50mL of 300 ppm metals (100ppm Pb, 100ppm Zn, 100ppm Cu)
10.58 mg CuCl2
10.57 mg ZnCl2
7.85 mg PbAc
50mL H2O
• Dilution to 60ppm
10mL of 300ppm and 40mL water
• 20% column data:

Dry:15.5904g

Wet: 15.9570g

• 10% column data

Dry:15.966g

Wet: 16.389g

Ran data analysis on 10% beads with MG

• Found the Max Absorbance for each run.
• Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample.
• Created plot of concentration vs. Volume Eluted.
• Calculated the amount of MG added to the Column
54 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg
• Calculated the amount of MG eluted.
From samples 1-54:
5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
For sample 55:
1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
4mL*1L/1000mL*concentration of sample = mg of MG desorbed
For samples 56-88:
5mL*1L/1000mL*concentration of sample = mg of MG desorbed
• Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column.
• Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads.

## Data

Data and Results for 10% Column with MG

Graph of Concentration of MG vs. Volume of MG Eluted

Amount desorbed: 0.2879mg

Mass of Wet Beads in Column: 1.465g

Mass of Dry Beads in Column: 0.2683g

Mass of Clay in Column: 0.02683g

Capacity of Clay: 480.8 mg/g

Swelling for 10% clay is 446%

• 4.46=wet-dry/dry
• 4.46*dry = wet-dry
• 4.46dry+dry= wet
• 5.46dry = wet
• wet = 1.465g
• 1.465/5.46 = dry
• dry mass = 0.2683g