User:Dhea Patel/Notebook/Hemoglobin Project/2013/07/02

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Reaction of OPD and H2O2 with Mb Catalyst in H2O or MeOH (5°C-65°C)

  • The H2O trials were completed with Mb/Tris.
  • More buffered protein solutions were made
  • The MeOH trials with Mb/Citrate began.

Mb/Tris in H2O, Trial 2 Data

Mb Tris OPD H2O 5C WORKUP GRAPH.png Mb Tris OPD H2O 5C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 15C WORKUP GRAPH.png Mb Tris OPD H2O 15C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 25C WORKUP GRAPH.png Mb Tris OPD H2O 25C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 35C WORKUP GRAPH.png Mb Tris OPD H2O 35C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 45C WORKUP GRAPH.png Mb Tris OPD H2O 45C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 55C WORKUP GRAPH.png Mb Tris OPD H2O 55C SEQUENTIAL WORKUP GRAPH.png

Mb Tris OPD H2O 65C WORKUP GRAPH.png Mb Tris OPD H2O 65C SEQUENTIAL WORKUP GRAPH.png

Mb/Tris in H2O, Trial 3 Data

Mb Tris OPD H2O 5C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 5C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 15C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 15C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 25C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 25C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 35C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 35C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 45C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 45C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 55C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 55C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb Tris OPD H2O 65C WORKUP GRAPH Trial3.png Mb Tris OPD H2O 65C SEQUENTIAL WORKUP GRAPH Trial3.png

Mb/Citrate in MeOH, Trial 2 Data

Mb Citrate OPD MeOH 5C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 5C SEQUENTIAL WORKUP GRAPH Trial2.png

Mb Citrate OPD MeOH 15C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 15C SEQUENTIAL WORKUP GRAPH Trial2.png

Mb Citrate OPD MeOH 25C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 25C SEQUENTIAL WORKUP GRAPH Trial2.png

Mb Citrate OPD MeOH 35C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 35C SEQUENTIAL WORKUP GRAPH Trial2.png

Mb Citrate OPD MeOH 45C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 45C SEQUENTIAL WORKUP GRAPH Trial2.png

Mb Citrate OPD MeOH 55C WORKUP GRAPH Trial2.png Mb Citrate OPD MeOH 55C SEQUENTIAL WORKUP GRAPH Trial2.png

    • There was not enough protein to run the reaction at 65°C (was to run on 7/3)