User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/02/14

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Remains of the Day

  • Ran gels of TRFLP from yesterday (Fungus with ITS1F/ITS4 - 35 cycles with 48 degree annealing) and today (Bacteria with 27F/1492Rh - - 35 cycles with 50 degree annealing). The latter was a dirty reaction with lots of primer dimer type stuff and so needs to be redone.