User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/01/19

Applied Soil Microbial Ecology

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Remains of the Day Run gel of PCRs from yesterday and do ethanol precipitation of select samples Finish ethanol precipitation of DNA and start restriction for TRFLP (added 8 ul of roughly 50ng/ul each PCR product and cut overnight with 1 U [0.1 ul] of DdeI) Plan tomato experiment - get Eric to do the germination and stuff Take another crack at Brazil data - write email to Dangl if its sorted out statistics (discriminant analysis) on PCA - http://madang.ajou.ac.kr/~daphnia/SBB0608.pdf PCR amplification misses half of the diversity of microbes in soil - http://www.nature.com/ismej/journal/v3/n12/full/ismej200989a.html 63F creates significant bias and should get low annealing temp - http://onlinelibrary.wiley.com/doi/10.1016/S0378-1097%2803%2900218-0/pdf 63F is biased at higher temperatures, 27F is not - http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2007.00283.x/abstract;jsessionid=F3976AFA501D1155092D1A492ABEB5AA.d02t04