User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/08/05

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The Daily Grind

  • To include the spermosphere in the analysis as a potential source of bacteria, 5 seeds of each genotype was placed in 5 ml of the buffer/tween overnight at room temperature in a lightly closed 15 ml falcon tube. After 24 hours, seeds will be shaken, removed from the tube, and the buffer frozen for later DNA extraction.
  • Gonna have to use the Ultraclean DNA extraction kit from MoBio for some samples, cause 100 preps is not enough...