User:DavidRamos/Lab/2006-6-22

June 22nd

Morning

• Incubator works.
• Marine strain WH8102 came in the mail with SH media.
• Went to MIT to pick up PCC7942 and 6803 from Peter Weigele at MIT.
  • Told us WH8102 will be way too hard to grow. We probably will go with the freshwater strains.
  • MIT food trucks go!

Afternoon

• Made 1L of the following liquid media:
  • 20 mL 50x BG-11 stock
  • 980 mL deionized H20
• Made 6 125ml liquid cultures in 250ml flasks:
  1. PCC7942 streak w/toothpick
  2. PCC7942 single w/toothpick
  3. PCC7942 single w/o toothpick
  4. PCC6803 streak w/toothpick
  5. PCC6803 single w/toothpick
  6. Control w/ toothpick
  • 16 hours light each day
  • Put flask holders in incubator and set shaker.
  • Open lids (parafilm with holes) to allow air exchange.
  • 30°C