User:DavidRamos/Lab/2006-6-15
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June 15th
Morning
- Brainstorming with Pam
- Conduct ligation reaction
- 6uL insert, 2uL vector, 2uL buffer, 10uL other buffer
- 5 min incubation @ RT
- Conduct transformation
- 20mL cells for positive, negative, and exp. ea (top 10)
Afternoon
- Plate transformant cells on LB-CARB
- Brainstorming session in the afternoon
