User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/06/27

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χρόνος πέρασμα June 27th 2011

ABSTRACT

  • The experiment to check which growing condition was best was finished. We couldn't use the gas chromatographer because the one we wanted to use broke down.

  • Today I left Chlamydomonas reinhardtii cells growing in order to let them ready to proceed with the procotol to isolate their mRNA and amplify the genes HydEF and HydG. They were left in a 250 ml flask; 50 ml of TAP medium with 50 μl of Ampicilin at a concentration of 100 μg / ml. The flask was left under the same condition as the petri dishes with C. reinhardtii (25°C with a light above).
  • Paulina Alatriste, Melissa Molho and I finished the experiment with the Phaseolus vulgaris bat477 inoculated with the different R. etli strains. As the gas chromatrographer we had planned to use broke down, we couldn't be able to continue as planned, that is, to measure the different components of the atmosphere of each flask to use it as a point of reference once we have our plants with the Rhizobia with our constructions. The only thing we could do was to measure the plants themselves; to see if it had any difference to let them grow in the culture room or in the greenhouse.


At Fede's greenhouse:

Plant # Nodules Nodule color # Leaves Leaves color Plant height
H1H2 2 10 10 white 2 1 green - 1 sick 22 cm
H1H2 5 42 42 white 4 2 green - 2 sick 27.5 cm
H1H2 3 17 17 white 4 2 green - 2 sick 28 cm
M4 4 37 7 stained brown - 30 white 4 2 green - 2 sick 27.5 cm
M4 3 62 18 stained brown - 44 white 4 2 green - 2 sick 28 cm
M4 1 38 38 white 4 2 green - 2 sick 27 cm
CFNX4 1 40 40 white 4 2 green - 2 sick 26 cm
CFNX4 6 51 4 stained brown - 47 white 4 2 green - 2 sick 29 cm
CFNX4 4 28 4 stained brown - 24 white 4 2 green - 2 sick 28 cm
CE3 wt 4 32 10 red - 22 white 4 2 green - 2 sick 25 cm
CE3 wt 2 41 7 stained brown - 34 white 4 2 green - 2 sick 25 cm
CE3 wt 3 29 14 stained brown - 15 white 4 2 green - 2 sick 25 cm
Control bat477 1 0 no nodules 4 2 green - 2 sick 30 cm
Control bat477 5 0 no nodules 4 2 green - 2 sick 30.5 cm
Control bat477 3 0 no nodules 4 2 green - 2 sick 24 cm
  • All plants but the H1H2 2 had four leaves, two old and sick, one trifolium like, and one sprout; these last two were taken as two green leafs.


At Esperanza's culture room:

Plant # Nodules Nodule color # Leaves Leaves color Plant height
H1H2 1 47 47 white 1 1 green 26.5 cm
H1H2 6 32 32 white 3 2 green - 1 yellow 20.5 cm
H1H2 4 66 Most white, some stained brown 2 2 green 26 cm
M4 2 20 13 white - 7 stained brown 3 3 green 18 cm
M4 6 154 41 white - 13 stained brown 2 2 green 26 cm
M4 5 108 54 white - 64 stained brown 2 2 green 27 cm
CFNX4 5 34 30 white - 4 stained brown 2 2 green 25 cm
CFNX4 2 74 74 white 3 3 green 24 cm
CFNX4 3 94 62 white - 32 stained brown 2 2 green 29 cm
CE3 wt 6 78 78 white/wrinkled 3 2 green - 1 sick 21 cm
CE3 wt 1 60 53 white - 7 stained brown 2 2 green 23.5 cm
Control bat477 6 0 no nodules 3 2 green - 1 sick 23.5 cm
Control bat477 4 0 no nodules 1 1 green 25 cm
Control bat477 2 0 no nodules 3 2 green - 1 sick 25.5 cm
  • Plant CE3 wt 6 was included in the experiment two days after all the other plants had grown enough (10/06/11). Plant CE3 wt 5 was not included at all because it did not grow.