χρόνος πέρασμα June 27th 2011
ABSTRACT
- The experiment to check which growing condition was best was finished. We couldn't use the gas chromatographer because the one we wanted to use broke down.
- Today I left Chlamydomonas reinhardtii cells growing in order to let them ready to proceed with the procotol to isolate their mRNA and amplify the genes HydEF and HydG. They were left in a 250 ml flask; 50 ml of TAP medium with 50 μl of Ampicilin at a concentration of 100 μg / ml. The flask was left under the same condition as the petri dishes with C. reinhardtii (25°C with a light above).
- Paulina Alatriste, Melissa Molho and I finished the experiment with the Phaseolus vulgaris bat477 inoculated with the different R. etli strains. As the gas chromatrographer we had planned to use broke down, we couldn't be able to continue as planned, that is, to measure the different components of the atmosphere of each flask to use it as a point of reference once we have our plants with the Rhizobia with our constructions. The only thing we could do was to measure the plants themselves; to see if it had any difference to let them grow in the culture room or in the greenhouse.
At Fede's greenhouse:
| Plant
|
# Nodules
|
Nodule color
|
# Leaves
|
Leaves color
|
Plant height
|
| H1H2 2
|
10
|
10 white
|
2
|
1 green - 1 sick
|
22 cm
|
| H1H2 5
|
42
|
42 white
|
4
|
2 green - 2 sick
|
27.5 cm
|
| H1H2 3
|
17
|
17 white
|
4
|
2 green - 2 sick
|
28 cm
|
| M4 4
|
37
|
7 stained brown - 30 white
|
4
|
2 green - 2 sick
|
27.5 cm
|
| M4 3
|
62
|
18 stained brown - 44 white
|
4
|
2 green - 2 sick
|
28 cm
|
| M4 1
|
38
|
38 white
|
4
|
2 green - 2 sick
|
27 cm
|
| CFNX4 1
|
40
|
40 white
|
4
|
2 green - 2 sick
|
26 cm
|
| CFNX4 6
|
51
|
4 stained brown - 47 white
|
4
|
2 green - 2 sick
|
29 cm
|
| CFNX4 4
|
28
|
4 stained brown - 24 white
|
4
|
2 green - 2 sick
|
28 cm
|
| CE3 wt 4
|
32
|
10 red - 22 white
|
4
|
2 green - 2 sick
|
25 cm
|
| CE3 wt 2
|
41
|
7 stained brown - 34 white
|
4
|
2 green - 2 sick
|
25 cm
|
| CE3 wt 3
|
29
|
14 stained brown - 15 white
|
4
|
2 green - 2 sick
|
25 cm
|
| Control bat477 1
|
0
|
no nodules
|
4
|
2 green - 2 sick
|
30 cm
|
| Control bat477 5
|
0
|
no nodules
|
4
|
2 green - 2 sick
|
30.5 cm
|
| Control bat477 3
|
0
|
no nodules
|
4
|
2 green - 2 sick
|
24 cm
|
- All plants but the H1H2 2 had four leaves, two old and sick, one trifolium like, and one sprout; these last two were taken as two green leafs.
At Esperanza's culture room:
| Plant
|
# Nodules
|
Nodule color
|
# Leaves
|
Leaves color
|
Plant height
|
|
| H1H2 1
|
47
|
47 white
|
1
|
1 green
|
26.5 cm
|
| H1H2 6
|
32
|
32 white
|
3
|
2 green - 1 yellow
|
20.5 cm
|
| H1H2 4
|
66
|
Most white, some stained brown
|
2
|
2 green
|
26 cm
|
| M4 2
|
20
|
13 white - 7 stained brown
|
3
|
3 green
|
18 cm
|
| M4 6
|
154
|
41 white - 13 stained brown
|
2
|
2 green
|
26 cm
|
| M4 5
|
108
|
54 white - 64 stained brown
|
2
|
2 green
|
27 cm
|
| CFNX4 5
|
34
|
30 white - 4 stained brown
|
2
|
2 green
|
25 cm
|
| CFNX4 2
|
74
|
74 white
|
3
|
3 green
|
24 cm
|
| CFNX4 3
|
94
|
62 white - 32 stained brown
|
2
|
2 green
|
29 cm
|
| CE3 wt 6
|
78
|
78 white/wrinkled
|
3
|
2 green - 1 sick
|
21 cm
|
| CE3 wt 1
|
60
|
53 white - 7 stained brown
|
2
|
2 green
|
23.5 cm
|
| Control bat477 6
|
0
|
no nodules
|
3
|
2 green - 1 sick
|
23.5 cm
|
| Control bat477 4
|
0
|
no nodules
|
1
|
1 green
|
25 cm
|
| Control bat477 2
|
0
|
no nodules
|
3
|
2 green - 1 sick
|
25.5 cm
|
- Plant CE3 wt 6 was included in the experiment two days after all the other plants had grown enough (10/06/11). Plant CE3 wt 5 was not included at all because it did not grow.
|
UNAM Genomics Mexico 2011
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