User:Daniel A. Vargas/Notebook/General lab notebook/2015/09/11

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Passaging of Hek293 cells The T-25 tissue culture flasks had all become 90% confluent and required passaging. Cells were passaged using a 1:5 ration in to new T-25 flasks and will be grown in these until it is time to transfer them into the 6 well plates for transfections.