User:Daniel A. Vargas/Notebook/General lab notebook/2015/09/04

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Dox induced Gal4-EED

Cells were thawed into a T-25 flask and a day later passaged into a T-75 flask with no dilution. HEK293 Gal4-EED-luc cells were allowed to grow for 2 more days until reaching 90% confluence, at this point the cells were passaged from the T-75 into 4 separate T-25 flasks that will be induced with dox for the selected hours. On day one the 192 hr plate was induced with 1microgram/milliliter of dox.
Plate Day of Dox ind.
192hrs 09/04/15
144hrs N/A
96hrs N/A
48hrs N/A
0 hrs N/A