User:Daniel A. Vargas/Notebook/General lab notebook/2015/07/15

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Plasmid Validation

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/Xbal/ApaL1/Bsa1 digests
Reagent 1 2 3-5 Volume Expected:
1. ANM5 = 4,261
2. MYB = 4,483
3. Carm1 = 4,418
4. KMT2E = 4,418
5. SETD1A = 7,715
Today's gel
30 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 3.0
EcoRI 1 0 1 1.0
Xbal 0 1 1 1.0
ApaL1 1 0 0 1.0
Bsa1 0 1 0 1.0
dH2O 20
  30 μL --> 37°C/ 15 min.