Plasmid Validation
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/Xbal/ApaL1/Bsa1 digests
Reagent
|
1
|
2
|
3-5
|
Volume
|
Expected: 1. ANM5 = 4,261 2. MYB = 4,483 3. Carm1 = 4,418 4. KMT2E = 4,418 5. SETD1A = 7,715
|
![Today's gel](https://s3-us-west-2.amazonaws.com/oww-files-public/e/ec/Insertverification.jpg) 30 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
|
|
|
3.0 μL
|
10X buffer |
|
|
|
3.0
|
EcoRI |
1 |
0 |
1 |
1.0
|
Xbal |
0 |
1 |
1 |
1.0
|
ApaL1 |
1 |
0 |
0 |
1.0
|
Bsa1 |
0 |
1 |
0 |
1.0
|
dH2O |
|
|
|
20
|
|
|
|
|
30 μL --> 37°C/ 15 min.
|
|