PCR amplification of chromatin remodeling
Reagent
|
Volume
|
Expected: 1. ANM5 = 1911, 2. MYB = 159,1764 3. Carm1 = 1827 4. SETD7 = 459,642 5. KMT2E = 1176,4401 6. SETD1A = 420,4704
|
Today's gel 15 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
1.0 μL
|
1X GoTaq |
12.5
|
F primer |
1.0
|
R Primer |
1.0
|
dH2O |
9.5
|
|
25 μL
|
Transforming P300 plasmids
Transformation protocol
Sample ID
|
Expected
|
Observed
|
colonies
|
PBS P300 |
Growth |
None |
0.00
|
NHA P300 |
Growth |
None |
0.00
|
CHA P300 |
Growth |
None |
0.00
|
Plasmids were sent on filter paper and is possible did not remain intact during transit.
|