User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29

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PCR amplification of chromatin remodeling

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Transforming P300 plasmids

Transformation protocol

Sample ID Expected Observed colonies
PBS P300 Growth None 0.00
NHA P300 Growth None 0.00
CHA P300 Growth None 0.00

Plasmids were sent on filter paper and is possible did not remain intact during transit.