User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/05/30
From OpenWetWare
Project name | Main project page Previous entry |
Gel ElectrophoresisWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
1. 10uL Sample Buffer + 10uL ladder 2. 10uL Sample Buffer + 1uL ladder + 9uL H2O 4. 10uL Sample Buffer + 10uL Supernatant 6. 10uL Sample Buffer + 1uL Supernatant + 9uL H2O 8. 10uL Sample Buffer + 0.1uL Supernatant + 9.9uL H2O 9. 10uL Sample Buffer + 1uL Supernatant + 9uL H2O 11. 10uL Sample Buffer + 1uL ladder + 9uL H2O 12. 10uL Sample Buffer + 10uL ladder
Protein PurificationYesterday's procedure was continued today |