- Remake dopamine coated AuNPs
- Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5
- Mix 17mL of AuNP solution with 17mL of the dopamine solution
- Stir under air at room temperature for 1 hour
- Purify by centrifuging and then redisperse in water.
- Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um
- Make 2 solutions.
- to be stored over night on lab bench
- to be stored over night in the refrigerator
- Testing Activity of Alpha-Method HRP:AuNPs
- A solution of H2O2 was prepared by mixing 113uL of 30% H2O2 with 887uL H20
- A solution of OPD was prepared by dissolving OPD in H20 to have a final concentration of 1mg/mL
- 10mg in 10mL
- In order to test the activity of the HRP:AuNP, the HRP:AuNPs were mixed with OPD and H2O2
- 0.75mL of H2O2, 2.025mL OPD and 0.225mL of sample
- The UV-Vis spectra showed that in the presence of the HRP:AuNP the OPD peak decreased and another peak appeared. Compared to a sample of OPD alone with H2O2, the sample with the nano particles showed the peak forming at a much faster rate.
- Because the solution may contain some free HRP molecules, we will centrifuge the solution to precipitate the nano particles coated with HRP and test them for activity.