User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/02/11

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Procedures Remake dopamine coated AuNPs Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5 Mix 17mL of AuNP solution with 17mL of the dopamine solution Stir under air at room temperature for 1 hour Purify by centrifuging and then redisperse in water. Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um Make 2 solutions. to be stored over night on lab bench to be stored over night in the refrigerator Testing Activity of Alpha-Method HRP:AuNPs A solution of H2O2 was prepared by mixing 113uL of 30% H2O2 with 887uL H20 A solution of OPD was prepared by dissolving OPD in H20 to have a final concentration of 1mg/mL 10mg in 10mL In order to test the activity of the HRP:AuNP, the HRP:AuNPs were mixed with OPD and H2O2 0.75mL of H2O2, 2.025mL OPD and 0.225mL of sample - The UV-Vis spectra showed that in the presence of the HRP:AuNP the OPD peak decreased and another peak appeared. Compared to a sample of OPD alone with H2O2, the sample with the nano particles showed the peak forming at a much faster rate. - Because the solution may contain some free HRP molecules, we will centrifuge the solution to precipitate the nano particles coated with HRP and test them for activity.