User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2011/10/18

Biomaterials Design Lab

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Entry title Objective Make a labeled cysteine fluorophore. Description Measure out 5mg of 5-(Iodoacetamido)fluorescein and put in a 50mL Falcon tube. Add 19.6μL of 118mg/mL cysteine to the 50mL Falcon tube. Add 35mL of 50mM Tris, pH 7.5. Add 100μL of 2.25M DTT after about two hours of the reaction occurring at room temperature. Data Add data and results here... Notes Abs at 490nm = 1.144AU for 20x dilute A=bcε, b=1cm, ε=42000M-1cm-1 c=27.24μM for 20x dilute actual concentration is 544.76μM concentration was supposed to be 277.231μM (.005g/(515.3g/mol)/(.035L)*106) Concentration of cysteine = ((118g/L)*(1.96*10-5L))/(121.16 g/mol)/(.035L)*106 = 545.395μM Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.