User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/09/06

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9/6/2016 Flourescence

1. Sample was made in a capped cuvette:

  1. pH 4
    1. Myoglobin: 0.25mM Au, 12.5μM Myoglobin, 3mL total
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 464 μL Myoglobin
      4. 1871 μL Water


  • Concentration of AuCl3stock was 5.08 mM so concentratio of AuCl3 in final solution was 0.381 mM NOT 0.25mM.
  • Concentration of Myoglobin was 183 uM so concentration of Myoglobin in final solution was 28.304 μM NOT 12.5 μM.


2. The cuvette was heated to 80C and samples were taken every 5 minutes using the fluorescence spectrometer for three hours.


3. Samples for future UV-Vis spectroscopy were made using prepared stock solutions:

stock solutions from: Dr. Hartings' Lab Notebook 08/29/2016

Volumes used in samples:

0 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Myoglobinstock μL of Water
4 500 0 0 500 0 342 3658
5 50 0 0 500 0 342 4108
6 5 0 0 500 0 342 4153
7 0 0 0 500 0 342 4158
8 0 5 0 500 0 342 4153
9 0 50 0 500 0 342 4108
10 0 500 0 500 0 342 3658
11 0 0 5 500 0 460 4153
12 0 0 50 500 0 460 4108


0.624 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Myoglobin stock μL of Water
4 500 0 0 500 240 342 3418
5 50 0 0 500 240 342 3868
6 5 0 0 500 240 342 3913
7 0 0 0 500 240 342 3918
8 0 5 0 500 240 342 3913
9 0 50 0 500 240 342 3868
10 0 500 0 500 240 342 3418
11 0 0 5 500 240 342 3913
12 0 0 50 500 240 342 3868


  • Concentration of AuCl3stock was 5.08 mM so final concentration of AuCl3 was 0.508 mM in sample NOT 0.25 mM.


4. Results

  • Wavelength

WavelengthCH09072016AU.png

  • Integrated Intensity

IntensityCH09072016AU.png

  • First 15 Minutes

First15CH09072016AU.png

  • 35-45 Minutes

35-45CH09072016AU.png


User:Matt Hartings/Notebook/AU Biomaterials Design Lab