User:Carly M. Montanero/Notebook/CHEM-571/2014/02/26

Biomaterials Design Lab: Spring 2014

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Objective Record results of 30:1 lysozyme:AuNP (with variables added after baking) that were heated at 37°C overnight. Take UV-Vis of samples with salt added after baking heated at 37°C overnight. Synthesize 30:1 lysozyme-AuNP solutions. Synthesize 60:1, 70:1, 80:1, 90:1, and 100:1 BSA-AuNPs baked with MgCl2 for 4 hours at 80°C. Procedure Synthesizing Lysozyme-AuNP and BSA-AuNP The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs. One tube of each ratio was made for BSA-AuNPs. For lysozyme-AuNPs, 25 tubes were made. The samples were heated in the oven at 80°C for 4 hours. Figures UV-Vis of Heated Samples and Variables (36°C) Notes These results detail the same samples as seen below; however, these samples were at 37°C before observing. After observations were taken, the samples were placed in the fridge. These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. The samples were at room temperature (37°C) when observations were taken.