User:Carly M. Montanero/Notebook/CHEM-571/2014/02/04

Biomaterials Design Lab: Spring 2014

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Objective Synthesize lysozyme-AuNP. Create stock solutions of chelators. Add the various chelators to lysozyme-AuNP solutions. Change pH of lysozyme-AuNP solutions. Record results. Procedure Synthesizing Lysozyme-AuNP Creating the Gold Stock Solution Add 0.0524 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water. The final concentration of gold stock was 2.66 mM. Creating the Lysozyme Stock Solution Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water. The final concentration of lysozyme stock was 100.28 μM. Creating Au:Protein Ratios of 40:1 and 50:1 For the 40:1 ratio, add 470 μL of gold solution, 312 μL of lysozyme solution, and 4218 μL of deionized water to a test tube. For the 50:1 ratio, add 470 μL of gold solution, 249 μL of lysozyme solution, and 4281 μL of deionized water to a test tube. We made 10 tubes of each ratio. The samples were heated at 80°C for 4 hours. Chelators Creating Stock Solutions of Chelators Adding Chelators to Lysozyme-AuNP Solutions Add 0.5 mL of each chelator stock solution to each 1.5 mL aliquot. Record effects of each chelator on each solution. 2,2 bipyridine was used instead of 2,2 bipyridyl pH Adding Buffers to Lysozyme-AuNP Solutions Dilute the 1 M solutions to 100 mM, 10 mM, and 1 mM. Add 1 mL of each to the lysozyme-AuNP solutions. Let sit overnight and record results.