||ISIS Neutron Facility
STFC Rutherford Appleton Laboratory
Harwell Science and Innovation Campus
Didcot, OX11 0QX, UK
|School of Chemistry|
University of Southampton
SO17 1BJ, UK
|I am a Senior Scientist at the ISIS Pulsed Neutron Source, Rutherford Appleton Laboratory, near Oxford with a joint appointment as a Lecturer in Chemical Biology at the School of Chemistry at the University of Southampton. The group at Southampton works in the areas of protein-ligand binding [1, 2, 3] and directed evolution  with a focus on methods development for high throughput analysis and protein labeling [5, 6]. We have recently developed methodology for the immobilisation of proteins on solid supports  as well as the site selective fluorescent labelling of proteins. I am also involved in the 4G Basic Technology project which is developing encoded microparticles for use in high throughput assays. The project has demonstrated the feasibility of encoding manufactured particles using minature diffraction gratings, the ability to carry out multistep synthesis on these particles , as well as performing multiplexed immunoassays and DNA hybridisation. We have also analysed the ability of high throughput DNA sequencing  to deliver useful information and developed new tools for designing hybridisation probes for DNA analysis .
At the Rutherford Appleton Laboratory the ISIS Biomolecular Sciences Group is developing and promoting neutron and X-ray scattering techniques for applications in the biological sciences. Scattering techniques provide a means of obtaining low resolution information on biomolecules in solution. It is particularly useful in determining the structure of biomolecular complexes in solution, especially where high resolution structural data is available on the individual components of the complex.
The combination of high throughput methodology and working on two sites has led to a developing programme in e-science particularly in the area of electronic lab notebooks. In collaboration with the e-science group and Professor Jeremy Frey at Southampton we are developing and testing an electronic notebook based on a blog. This has the potential to provide a very flexible framework for recording and analysing data. This is currently at an early stage of development but the work in progress can be viewed here (Blog view - Timeline view - RSS Feed).
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- Methods for selective protein labelling
- Directed evolution
- High throughput methods for genotype and phenotype analysis
- Small angle X-ray and Neutron scattering
- Electronic lab notebooks and automatic data capture
- Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, and Dixon NE. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006 Jun 30;125(7):1309-19. DOI:10.1016/j.cell.2006.04.040 |
- Neylon C, Kralicek AV, Hill TM, and Dixon NE. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005 Sep;69(3):501-26. DOI:10.1128/MMBR.69.3.501-526.2005 |
- Neylon C, Brown SE, Kralicek AV, Miles CS, Love CA, and Dixon NE. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000 Oct 3;39(39):11989-99.
- Neylon C. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution. Nucleic Acids Res. 2004;32(4):1448-59. DOI:10.1093/nar/gkh315 |
- Wood RJ, Pascoe DD, Brown ZK, Medlicott EM, Kriek M, Neylon C, and Roach PL. Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem. 2004 Mar-Apr;15(2):366-72. DOI:10.1021/bc0341728 |
- Chan L, Cross HF, She JK, Cavalli G, Martins HF, and Neylon C. Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation. PLoS One. 2007 Nov 14;2(11):e1164. DOI:10.1371/journal.pone.0001164 |
- Cavalli G, Banu S, Ranasinghe RT, Broder GR, Martins HF, Neylon C, Morgan H, Bradley M, and Roach PL. Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single material. J Comb Chem. 2007 May-Jun;9(3):462-72. DOI:10.1021/cc060079p |
- Whiteford N, Haslam N, Weber G, Prügel-Bennett A, Essex JW, Roach PL, Bradley M, and Neylon C. An analysis of the feasibility of short read sequencing. Nucleic Acids Res. 2005 Nov 7;33(19):e171. DOI:10.1093/nar/gni170 |
Weber G, Haslam N, Whiteford N, Prügel-Bennett A, Essex JW, and Neylon C. Thermal equivalence of DNA duplexes without calculation of melting temperature. Nature Physics, 2(1), 55-59, doi:10.1038/nphys189