User:Brian P. Josey/Notebook/2010/02/09

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Yesterday

I didn't come in yesterday because I have a paper for my history class that was giving me grief. I took yesterday to go through everything that I needed to do to get the paper taken care of and out of my hair.

Tomorrow

So tomorrow, I want to try two things. The first is that I want to try diluting the ferritin, and seeing if I can move it in the tube. On Friday, when I was playing with the magnetic beads, I noticed a couple of things:

  1. . When I mixed them up, the tube became cloudy as the beads when into solution from mixing.
  2. . Placing a magnet next to the tube made the solution more clear.
  3. . Leaving the magnet next to the tube for a long time, about an hour, makes the tube completely clear, and all of the beads clump together as close to the magnet as possible.

I doubt that we can see any change, but it would be worth it to at least try. So I am going to create a dilution, and set it up next to either a magnet, the yoke, or both and see what happens.

The second thing that I want to try, is seeing if I can see anything through the microscope. I know that the proteins are very small, and the beads that we looked at were very large, but I am curious to find out if we can see them. If not, is there a way that we can see them, and so forth.

I also need to go through the magnet dealer's website, to see what we can do about a neodymium yoke, and if I can't find anything useful, I need to check out what a custom job would cost. Steve Koch 22:40, 9 February 2010 (EST): A couple notes:

  • It's probably worth getting the neodymium conical tips (assuming they're higher sat mag than iron and not too pricey)
  • As we mentioned, dark field may be good for viewing the ferritin. A disadvantage, though is that you'll have a condenser, which gets in the way of the magnet! If a bunch of it aggregates near a conical tip, you may be able to see it via microscopy, even if you can't discern individual particles.
  • Good luck!