User:Bill Flanagan/Sandbox

From OpenWetWare

My Images

OD600vsCellDensity.JPG:
   
File:OD600vsCellDensity.JPG
OD600 vs. CFU density
2005.4.5-2.jpg:
   
File:2005.4.5-2.jpg
Closeup of pump with cells
Headbg.jpg:
   
File:Headbg.jpg
banner
KlettvsCellDensity.png:
   
File:KlettvsCellDensity.png
Calibration Curve for Klett vs. Cell Density
Seq1.jpg:
   
File:Seq1.jpg
seq of library
Spec calibration.xls:test of excel upload
SSchemo.PNG:
   
File:SSchemo.PNG
early chemostat results
MRFP1.JPG:
   
File:MRFP1.JPG
mRFP1 spectra
2005.06.07.ChemostatRun.JPG:
   
File:2005.06.07.ChemostatRun.JPG
06.07.2005 Chemostat Run
050607Chemo12hr.PNG:
   
File:050607Chemo12hr.PNG
06.07.2005 Chemostat Run
ChemoIP1 orig.jpg:
   
File:ChemoIP1 orig.jpg
chemostat image process original
ChemoIP1 orig bright.jpg:
   
File:ChemoIP1 orig bright.jpg
Chemostat IP orignal brightened
ChemoIP1 process.jpg:
   
File:ChemoIP1 process.jpg
chemostat image process processed
CHR41028.PNG:
   
File:CHR41028.PNG
41028 Chroma Spectra
CHR41001.PNG:
   
File:CHR41001.PNG
Chroma spectra 41001
CHR31044v2.gif:
   
File:CHR31044v2.gif
Chroma spectra 31044v2
CHR41043.gif:
   
File:CHR41043.gif
Chroma spectra 41043
90hrRunf.PNG:
   
File:90hrRunf.PNG
90hr chemostat run
BEING-leaf-logo.jpg:
   
File:BEING-leaf-logo.jpg
Logo for BEInG front page
Bioreactor-schematic.jpg:
   
File:Bioreactor-schematic.jpg
Schematic of a hydrogen photobioreactore (BEInG)
Luciferyeast.JPG:
   
File:Luciferyeast.JPG
luciferyeast
DNANanodropExample1.PNG:
   
File:DNANanodropExample1.PNG
Example of wavelength vs. absorbance curve for a miniprep sample on the nanodrop.
Anthropology.PNG:
   
File:Anthropology.PNG
anthropolgy home page pic
GFPstandardProcess.PNG:
   
File:GFPstandardProcess.PNG
process for GFP measurement standardization work
Streak plates.png:
   
File:Streak plates.png
Agar plate being streaked with bacteria. Drawn by Theresa Knott. thanks wikipedia!
BEBoard.JPG:
   
File:BEBoard.JPG
BE Board Logo
BEInG lab.jpg:
   
File:BEInG lab.jpg
Setup for BEInG Hydrogen Bioreactor 9/26/2005
Changing Sensor Zero Values.pdf:document for changing sensor zero values on solent incubator.
JK ICSB 2005.pdf:Jason Kelly ICSB 2005 Poster
SortoStatDesign.PNG:
   
File:SortoStatDesign.PNG
Sortostat design image
GrowthIssues.JPG:
   
File:GrowthIssues.JPG
Graph of cells dying out insortostat in long runs.
MOFLObeads.PNG:
   
File:MOFLObeads.PNG
Example of what Spherotech RCP-60-5 Rainbow Calibration Particles (6.0-6.4um) should look like on the MOFLO under good alignment.
TestImage.PNG:
   
File:TestImage.PNG
test Image
MOFLODarkNoise.jpg:
   
File:MOFLODarkNoise.jpg
Example of "dark noise" on non-flourescent cells on the MOFLO as a result of high PMT settings. (A) FL1=max / FL7=max (B) FL1 = 525 / FL7=max (C) FL1=525 / FL7 = 650.
UT HelloWorld.jpg:
   
File:UT HelloWorld.jpg
UT austin biofilm from wikipedia
ScreeningPlasmidDesign.PNG:
   
File:ScreeningPlasmidDesign.PNG
Schematic and actual design for the screening plasmid
ScreeningPlasmidSchematic.PNG:
   
File:ScreeningPlasmidSchematic.PNG
Schematic of Screening Plasmid
Q04740sp.PNG:
   
File:Q04740sp.PNG
q04740 characterization with SP1.0
ScreeningPlasmid1.0.PNG:
   
File:ScreeningPlasmid1.0.PNG
Screening Plasmid 1.0
BeingalgaeSetup.jpg:
   
File:BeingalgaeSetup.jpg
BEInG's Setup for growing algae to make H2.
JK BE Retreat 2006.pdf:BE Retreat 2006 poster
TermsCorrected.jpg:
   
File:TermsCorrected.jpg
Terminator characterization with Screening Plasmid 1.0
TermDotPLot.JPG:
   
File:TermDotPLot.JPG
Dot plot of terminators characterizaed by screening plasmid 1.0
JK.SyntheticBiology2.0.Poster.pdf:Jason Kelly's Synthetic Biology 2.0 Poster
PACCAR16.JPG:
   
File:PACCAR16.JPG
pACCAR16 structure
OpenWetWare iCampus Spring Review.pdf:OpenWetWare Spring Review for iCampus Grant
DelLacYInduction.PNG:
   
File:DelLacYInduction.PNG
Induction of lac-regulated promoter in lacY- and lacY+ strains, from Jensen et al. Eur.J.Biochem, 211, 181-191 (1993)
MFruit Curves.bmp:
   
File:MFruit Curves.bmp
this is a test
I13534vsI13457.JPG:
   
File:I13534vsI13457.JPG
another test
EndyCirculatorManual.pdf:Manual for the Polyscienec Circulator.
ICampus-SpringReview-FINAL.doc:Word version of the OWW iCampus spring review for backup.
July8 2006 gel.jpg:
   
File:July8 2006 gel.jpg
Icampus.PNG:
   
File:Icampus.PNG
MIT iCampus Logo
Csbi.PNG:
   
File:Csbi.PNG
MIT CSBI logo
Jkpic.JPG:
   
File:Jkpic.JPG
be directory photo
OWW tshirt.jpg:
   
File:OWW tshirt.jpg
OWW tshirt option
MFruit Curves.PNG:
   
File:MFruit Curves.PNG
mFRuit curves with 568nm laster line approximately positioned on the graph. Image comes from Shaner et al, Nature Biotech, Nov 2004.
Endy NikonTE2000.jpg:
   
File:Endy NikonTE2000.jpg
Picture of Endy Lab Microscope
Nicholson1997Fig1.PNG:
   
File:Nicholson1997Fig1.PNG
Figure 1 from Zhang and Nicholson, PNAS 1997. (A) Substrate alignment analysis. Ten substrates were aligned whose cleavage sites were accurately determined, either by direct RNA sequence analysis or by primer extension of RNA cleaved in vitro by purified RNase III. (B) The “disfavored” bp, displayed in a dsRNA structure. The proximal box (PB) and distal box (DB) are included within an 11-bp helix. S = C or G, with S′ complementary to S. N, N′ indicate complementary nucleotides; while n, n′ indicate less strict complementarity. Arrowheads indicate the (blocked) cleavage sites. (C) Absence of conservation (degeneracy) of RNase III substrate sequence. H = A, G, U, with D′ (A, C, U) complementary to H; B = C, G, U, with V′ (G, C, U) complementary to B; W, W′ = A, U. (D) Secondary structure of the T7 R1.1 RNase III substrate, showing the proximal and distal boxes and the single cleavage site (arrowhead).
Fig1-becskei2000.PNG:
   
File:Fig1-becskei2000.PNG
figure 1 from becskei and serrano, nature 2000.
Nature Presentation - Feb5-07.ppt:Presentation to NPG NY office on Feb 5, 2007 by Sri, Jason, and Drew.
OWW round.jpg:
   
File:OWW round.jpg
Higher resolution copy of the sticker.
702px-I13600 - UV 254nm.jpg:
   
File:702px-I13600 - UV 254nm.jpg
Valves-partialClosed.PNG:
   
File:Valves-partialClosed.PNG
partially closed valves
PromoterChar Report Revised.doc:doc on promoter char by Caitlin Conboy
JK.BERetreat.Poster.2007.pdf:Poster presented by Jason Kelly at MIT BE retreat 3/2007
Weiss-sbreview-f2.jpg:
   
File:Weiss-sbreview-f2.jpg
Figure 2 from Andrianantoandro et al MSB 2006 (permission granted for re-use from NPG and EMBO).
Weiss-sbreview-f1.jpg:
   
File:Weiss-sbreview-f1.jpg
Figure 1 from Andrianantoandro et al MSB 2006 (permission granted for re-use from NPG and EMBO).
Example.jpg:
   
File:Example.jpg
DNAonly vertical.png:
   
File:DNAonly vertical.png
shirt with no circle around logo
DNA only horizontal.png:
   
File:DNA only horizontal.png
no circle around emblem horizontal
MofloBEADS-070520.png:
   
File:MofloBEADS-070520.png
Example of what Spherotech RCP-60-5 Rainbow Calibration Particles (6.0-6.4um) should look like on the MOFLO under good alignment. 05/20/07
MntInverter.png:
   
File:MntInverter.png
Mnt-based inverter in SP1.0
Q04400InverterInSP1.0.PNG:
   
File:Q04400InverterInSP1.0.PNG
Q04400 in SP1.0
Q04400LibInSP1.0.PNG:
   
File:Q04400LibInSP1.0.PNG
Q04400 library in Sp1.0
Q04401InSp1.0.PNG:
   
File:Q04401InSp1.0.PNG
Q04401 (mutant isolate from Q04400 library) in SP1.0
Sortostat v2.0.PNG:
   
File:Sortostat v2.0.PNG
Autocad image of Sortostat v2.0
Sortostat v2.0 - whiteBG.JPG:
   
File:Sortostat v2.0 - whiteBG.JPG
AutoCAD image of the Sortostat v2.0 design on white background with layers colored (blue = fluid, red = push UP, green = push DOWN)
Sortostat-valves-partialClosed.PNG:
   
File:Sortostat-valves-partialClosed.PNG
Example of valves not sealing properly due to fabrication problems.
IGEM-cropped-edited.PNG:
   
File:IGEM-cropped-edited.PNG
picture of MIT home page w 2006 iGEM team on it
CFP-YFP plate reader comparison.PNG:
   
File:CFP-YFP plate reader comparison.PNG
Comparison of CFP and YFP expressing cells in the plate reader.
07.06.13.jpg:
   
File:07.06.13.jpg
Gel image
IGEM06-Mint.JPG:
   
File:IGEM06-Mint.JPG
iGEM results for mint
JK.SB3.0.Poster.2007.pdf:Jason Kelly SB3.0 Poster
070909-pcr assembly.jpg:
   
File:070909-pcr assembly.jpg
PCR fusion test gel
070909-pcr assemblyB.jpg:
   
File:070909-pcr assemblyB.jpg
ESP-promoterTest.PNG:
   
File:ESP-promoterTest.PNG
Results of experiment by Jason Kelly to look at possible promoter in the empty screening plasmid
ESP-hairpinTest.PNG:
   
File:ESP-hairpinTest.PNG
Experiment to test hairpin effectiveness in the empty screening plasmid.
Patent squad memo-Breetz-1.doc:Memo by Hanna Breetz evaluating the viability of the patent goon squad
Ecoli.jpg:
   
File:Ecoli.jpg
A plush microbe from Giant Microbes.com
LacI-MullerHill94.JPG:
   
File:LacI-MullerHill94.JPG
Figure 1 from Muller-Hill et al. EMBO J. 1994 July 15; 13(14): 3348–3355.
OWW-mithomepage.PNG:
   
File:OWW-mithomepage.PNG
OpenWetWare on the MIT homepage
BarberoECDimage.JPG:
   
File:BarberoECDimage.JPG
Image from Robbie Barbero, Grad student at MIT, from virus-based electrochromic devices that can change from clear to opaque when a charge is applied.
FreeGenes.JPG:
   
File:FreeGenes.JPG
Psb3k3 Pcr prep.JPG:
   
File:Psb3k3 Pcr prep.JPG
preparatory PCR of psb3K3 on gel
RBS tester colony PCR.JPG:
   
File:RBS tester colony PCR.JPG
Colony PCR of 15 colonies following a construction using the RBS Measurement Kit
MeasKit inputs.JPG:
   
File:MeasKit inputs.JPG
inputs to the measurement kit run on a gel to verify length and digestion
Sortostat f2620.PNG:
   
File:Sortostat f2620.PNG
SynBERC talk Reisinger.ppt:Sarah Reisinger Talk for SynBERC slides from Leonard Katz
GFP RBS relative dgfp 080204c.jpg:
   
File:GFP RBS relative dgfp 080204c.jpg
RBS tester
Promoter tester demo.jpg:
   
File:Promoter tester demo.jpg
Variety of constitutive promoters inserted into the promoter measurement kit.
Measkit promoter FACS.PNG:
   
File:Measkit promoter FACS.PNG
Torch-01-june.gif:
   
File:Torch-01-june.gif
torch
Promoter table.JPG:
   
File:Promoter table.JPG
from Hawley & McClure, NAR Vol 11 (8) 1983 Table of nucleotide frequencies in the consensus e.coli sigma70 promoter
PromotersInReg.JPG:
   
File:PromotersInReg.JPG
Proposed promoter standard.JPG:
   
File:Proposed promoter standard.JPG
proposed promoter standard with fixed +1 site
Proposed promoter standard2.JPG:
   
File:Proposed promoter standard2.JPG
another option for BB promoter standard.
Oww onOLPC.jpg:
   
File:Oww onOLPC.jpg
OWW on the OLPC XO computer
Harris-mage.PNG:
   
File:Harris-mage.PNG
harris presents MAGE
Repressor sites.gif:
   
File:Repressor sites.gif
Moss1.jpg:
   
File:Moss1.jpg
Moss2.jpg:
   
File:Moss2.jpg
Moss5.jpg:
   
File:Moss5.jpg
Moss3.jpg:
   
File:Moss3.jpg
Ginkgologo.PNG:
   
File:Ginkgologo.PNG
JKM Glycerols.xls:JKM glycerols from endy lab
KC Glycerols.xls:KC glycerols