User:Benjamin Hirschi

I am a new member of OpenWetWare!

Contact Info

Benjamin Hirschi (a cartoonized interpretation)

• Benjamin Hirschi
• Helmholtz Center Munich
• Institute of Stem Cell Research
• Ingolstädter Landstr. 1
• D-85764 Neuherberg, BY, Germany
• Email me through OpenWetWare

I work in the Scheel lab focusing on mammary stem cells and breast cancer at the Helmholtz Center Munich (German Research Center for Environmental Health). My main project is to establish protocols for immortalization and transformation of primary cells from healthy donors using pathophysiologically relevant genetic events (i.e., mutations that do occur in breast cancer).

Education

• 2012, PhD, LMU Munich (Germany), University Hospital Munich, Department of Internal Medicine II
• 2006, Diploma, WWU Münster (Germany), Institute of Biochemistry

Research interests

1. Cancerogenesis in breast cancer: Are the different subtypes of breast cancer determined by the cell of origin, or subtype-specific genetic events ... or maybe both?
2. Oncogenic transformation: Can primary cells from healthy donors get in vitro-transformed into cancer cells by applying physiological mechanisms? And will transformation happen in a 2-step process (i.e., immortalization and subsequently oncogenic transformation)?
3. Cell line establishment: Can we create isogenic cell lines in order to compare immortalized primary cells to their corresponding oncogenic nemesis?
4. Breast cancer models: Will such corresponding/isogenic cell lines be suitable to model carcinogenesis in situ when cultivated in a 3D environment?

Publications

1. Hirschi B and Kolligs FT. Alternative splicing of BRAF transcripts and characterization of C-terminally truncated B-Raf isoforms in colorectal cancer. Int J Cancer. 2013 Aug 1;133(3):590-6. DOI:10.1002/ijc.28061 |

   PhD project

2. Schneider MR, Dahlhoff M, Horst D, Hirschi B, Trülzsch K, Müller-Höcker J, Vogelmann R, Allgäuer M, Gerhard M, Steininger S, Wolf E, and Kolligs FT. A key role for E-cadherin in intestinal homeostasis and Paneth cell maturation. PLoS One. 2010 Dec 14;5(12):e14325. DOI:10.1371/journal.pone.0014325 |

   PhD side show project

3. Wedeken L, Ohnheiser J, Hirschi B, Wethkamp N, and Klempnauer KH. Association of Tumor Suppressor Protein Pdcd4 With Ribosomes Is Mediated by Protein-Protein and Protein-RNA Interactions. Genes Cancer. 2010 Mar;1(3):293-301. DOI:10.1177/1947601910364227 |

   containing results of my diploma thesis

Useful links

• Introductory tutorial
• OpenWetWare help pages