Date
|
Plasmid Conc.
|
Therm Cycling Conditions
|
Gel Picture
|
Comment
|
11/9/2013 |
9 ng Plasmid: BD002 |
[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/cf/20131109_123553_%282%29.jpg/250px-20131109_123553_%282%29.jpg) |
|
11/9/2013 |
9 ng Plasmid: KAH187 (CMV) |
[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/7e/20131109_123553_%283%29.jpg/250px-20131109_123553_%283%29.jpg) |
|
11/10/2013 |
0.09ng Plasmid: BD002 |
[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/a/aa/20131110_122904_%282%29.jpg/250px-20131110_122904_%282%29.jpg) |
Gel Extract the band close to 5K bps No result
|
11/10/2013 |
0.09ng Plasmid: BD002 |
[95C (30 sec.), 45C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/cd/20131110_122904_%283%29.jpg/250px-20131110_122904_%283%29.jpg) |
Amplified with V0200 Sequencing Primers to make sure Kozak-HSV-CFP-NLS-Stop-PolyA (2K bps are there).
|
11/11/2013 |
Plasmid: BD002 |
[95C (30 sec.), 49C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/2/29/20131110_213024_%282%29.jpg/250px-20131110_213024_%282%29.jpg) |
Column 1,2: 9 ng Plasmid, Column 3,4: 0.09 ng Plasmid, Column 5,6: 0.5 ml primer from 10mM solution
|
11/11/2013 |
0.09ng Plasmid: BD002 |
[95C (30 sec.), 49C (45 Sec.), 75C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/fb/20131110_213024_%283%29.jpg/250px-20131110_213024_%283%29.jpg) |
Increase the extention temperature to 75C and annealing temperature to 49C
|
11/11/2013 |
0.09ng Plasmid: BD002 |
[95C (30 sec.), 55C (45 Sec.), 72C (6 min.)] |
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/48/20131111_095957_%282%29.jpg/250px-20131111_095957_%282%29.jpg) |
Increase the annealing temperature to 55C
|