- Pick 10 colonies of each plate and grow in 2 mL LB AMP broth for 6 hours.
- Spin down the cells for 3 minutes in microcentrifuge with top speed.
- Resuspend the pallet in 100μL dH2O.
- Incubate the cells in 98°C for 5 minutes.
- Spin down the cells for 3 minutes in microcentrifuge with top speed.
- Take the supernatent as DNA template for PCR reactions.
- PCR colonies at: 95°C for 3 min. , [95°C 3 min, 45°C 45 sec., 72°C 3 min.] ×35, 72°C 6min. 4°C ∞.
![BD004(HPK promoter) bands shows that only the promoter part is amplified (588bps)](https://s3-us-west-2.amazonaws.com/oww-files-thumb/6/6d/20131029_103655_%283%29.jpg/350px-20131029_103655_%283%29.jpg) |
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BD004(HPK promoter) bands shows that only the promoter part is amplified (588bps) |
BD003(CMV promoter) bands shows that only the promoter part is amplified (~ 600bps)
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