User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/10/18

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Golden Gate Assembly Confirmation

  • Growing three colonies of each plate in 6mL LB broth for 6 hrs.
  • Miniprep Plasmids and measure the concentration.
  • Concentrations are very low.
  • Cut the plasmids with SpeI and NotI, Not band were seen on the gel.