User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11

PcTF Subcloning in E. coli

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06/11/2013 Transfection Fluorescent Microscopy KAH187 BD002 Error creating thumbnail: Unable to save thumbnail to destination Error creating thumbnail: Unable to save thumbnail to destination Positive control,fluorescence was detected with Cyan filter. BD002, No fluorescence was detected in cells Fluorescence protein is in low concentration dueweak HSVtk TATA promoter, Next step: antibiotic colony selection technique Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection