User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11

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06/11/2013

Transfection Fluorescent Microscopy

KAH187 BD002

KAH187.jpg

BD002.jpg

  • Positive control,fluorescence was detected with Cyan filter.
  • BD002, No fluorescence was detected in cells
  • Fluorescence protein is in low concentration dueweak HSVtk TATA promoter,
  • Next step: antibiotic colony selection technique
  • Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection