14/12/2012
Ligation of KAH111 and C-His vector in NEB10B and BL21
Ligation |
Plate results (lig : neg crtl) 12/12/12
|
1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng |
KAH111/C-His 3:1 No Colonies
|
2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng |
KAH111/C-His 3:1 No Colonies
|
3. C-His(E/P)/ 25 ng |
|
|
1 |
2 |
3
|
Insert DNA |
3.12 |
3.12 |
---
|
Vector DNA |
2.94 |
2.94 |
2.94
|
2x lgn buf (Roche) |
7.06 |
7.06 |
5.0
|
T4 ligase (NEB) |
1.0 |
1.0 |
1.0
|
dH2O |
0 |
0 |
0.88
|
|
14.12 μL |
14.12 μL |
10μl
|
|