User:Arjun Naskar
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Registration/Questionnaire: 20.109 Fall 2007
Last Name
Naskar
First Name
Arjun
Preferred name
Arjun
Course/Minor
Major: 20 Minor: 14&15
Year of Graduation
2009
Telephone #
ANaskar AT mit DOT edu
Have you taken
7.05/5.07 (Biochemistry) yes
7.06 (Cell Biology) no
7.02 (General Biology Lab) no
5.310 (General Chemistry Lab) no
Do you have any experience culturing cells (mammalian, yeast or microbial)?
Yes No
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
Yes No
Please briefly describe any previous laboratory experience
I have worked in a UROP setting since fall of 2006. I am familiar with basic laboratory equipment and safety but have never dealt with biology-related lab work
Anything else you would like us to know?
n/a
Research Proposal Link
Arjun and Patrick's Research Proposal
M13 Re-Engineering Ideas
| Protein | Function | Re-engineering Ideas |
|---|---|---|
| I | Assembly | control channel/secretion to make it more selective |
| II | Replication of DNA + strand | make it so replication is faster and more efficient |
| III | Phage tail protein (5 copies) | make it so it has a more diverse contact site, not only TolA |
| IV | Assembly | see p1 |
| V | Binds ssDNA | increase amount of p5 to see how the rate of replication changes |
| VI | Phage tail protein (5 copies) | more copies of p6 to see if rate of infection is altered |
| VII | Phage head protein (5 copies) | attach GFP so we can know when phages are being secreted |
| VIII | Phage coat protein (2700 copies) | alter p8 to bind w/ other proteins, not only E.Coli |
| IX | Phage head protein (5 copies) | attach GFP |
| X | DNA replication | |
| XI | Assembly | see p11 |
