User:Anson Parker/Notebook/Enzymatic THC/Entry Base

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Overview The process for creating industrial quantities of THC is available from Biocyc.org What is needed is the specifics to producing Hexanoyl-CoA and Acetyl-CoA, Olivetolic Acid, Cannabigerolic Acid, and lastly Tetrahydrocannabigerolic Acid. In each case best parctices in terms of host organisms should be identified, as well as possible tricks and tweaks you the experts might note for optimizing or simplifying production. Extracting Enzymes Before cloning may begin the enzymes must be extracted and sequenced or synthesized from published sequences. For the sake of beginners we'll assume you need to do the extraction and sequencing from start to finish. Amplifying Sequences