User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/29

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Fluorescence Sample Preparation

The Fluorescence sample was prepared as follows: Calculation temp anneliesefaustino 113016.PNG

BSA Fluorescence

  1. pH 11
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 161108

  1. [BSA]= 30.4 uM
  2. [Au]= 3.37 mM
  3. [NaOH]= 1 M

Volumes for making the solution:

  1. 308.38 uL BSA
  2. 225.55 uL Au
  3. 2466.06 uL HOH
  4. 3.00 uL NaOH

Data Analysis

Fluorph11emissionamf.PNG

This chart shows the emission maximum of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The emission maximum increases at the very start of the experiment and remains level at around 420 nm for the remainder of the run. No nanoparticles form at pH 11, which is the reason that the emission maximum is relatively stable.

Fluorph11integratedamf.PNG

This chart shows the integrated intensity of the emission of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The integrated intensity rapidly increases at the start and then slowly increases over time.

Fluorph11scansparta.PNG

This chart shows the first few scans of emission intensity of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. As usual, the emission intensity increases rapidly for the first few scans and then does not increase much more. The next two charts show later scans, which remain at about the same emission intensity.

Fluorph11scanspartb.PNG

Fluorph11scanspartc.PNG