User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/01

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Entry title Insert content here... BSA Fluorescence Solution pH 9 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 160920 [BSA]= 52.8 uM [Au]= 2.64 mM [NaOH]= 1 mM Volumes for making the solution: 177.556 uL BSA 284.09 uL Au 2508.35 uL HOH 30 uL NaOH Matlab Notes Object: Peak alignment. Johnathan Newport for opening the comp c/users/physics admin/desktop HERE to open file: M=dlmread('goldfilename'); <---can tab to autocomplete cftool <-- open new window (curve fitting tool) X=M(:,1); <adding ; at end makes it not display> Y=M(:,2); cftool Peaks 328 353 385 438 488 Water raman peak X is x Y is y Gaussian, # terms ~4>look at a/b/c terms Fit options Full data Myo=dlmread('file') Xm=Myo(:,1); T0=Myo(:,2); T1=Myo(:,3); Do it for every timepoint. cftool X=Xm Y=To fit options>> Chance peak to redefine as gaussian peak we found above using a/b/c terms Write down peaks we think are correct based on broad/sharp peaks >try to set width. 353, 437 (important sharp peaks record) At final timepoint T29=Myo(:,32); Looks much different Fit options, set b1 to term 328 a1 start 5, lower 0, upper 10 >This is fitting it SO: Water Raman c term is 5-10 wide Others between 30-50 wide Five term gaussian to model 5 peaks: 328, 353, 385, 438, 488 35-40 wide c term +/- 5 on all but water raman (+/- 0.5) So b term gets +/- 5 on the given term at the inf parts The c term is 35-40 Fluorescence Data Analysis The chart above shows wavelength of the emission maximums for 3.125 uM BSA, 0.25 mM Au at pH 9 over time. The emission maximum holds relative steady, fluctuating by 5 nm above and below 420 nm for the entire length of the run. The chart above shows the integrated emission for 3.125 uM BSA, 0.25 mM Au at pH 9 over time. The integrated emission intensity begins at about 8000 at the start of the run. The integrated intensity increases to 11,500 at around 1200 s (forming a peak). The integrated emission intensity then decreases until about 5500 s (to a value of about 8500 intensity units), when it slowly begins to increase up to 11,000 intensity units until the end of the run at 12,000 s. The above chart shows the first 1000 s of emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. The emission intensity is seen to increase rapidly at first, then less slowly over time. The above chart shows 1000 s.to 4500 s. of emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. The emission intensity is seen to not increase much more after 1000 s. The above chart shows 4500 s to the end of the run of the emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. Again, the emission intensity is seen to not increase much more after 1000 s.