User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/01

From OpenWetWare
Jump to navigationJump to search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

  • Insert content here...


BSA Fluorescence Solution

  1. pH 9
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 52.8 uM
  2. [Au]= 2.64 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 177.556 uL BSA
  2. 284.09 uL Au
  3. 2508.35 uL HOH
  4. 30 uL NaOH

Matlab Notes

Object: Peak alignment.

Johnathan Newport for opening the comp

c/users/physics admin/desktop


HERE to open file:

M=dlmread('goldfilename'); <---can tab to autocomplete

cftool <-- open new window (curve fitting tool)

X=M(:,1);

<adding ; at end makes it not display>

Y=M(:,2);

cftool


Peaks 328 353 385 438 488


Water raman peak

X is x

Y is y

Gaussian, # terms ~4>look at a/b/c terms

Fit options


Full data

Myo=dlmread('file')

Xm=Myo(:,1);

T0=Myo(:,2);

T1=Myo(:,3);

Do it for every timepoint.


cftool

X=Xm

Y=To

fit options>> Chance peak to redefine as gaussian peak we found above using a/b/c terms


Write down peaks we think are correct based on broad/sharp peaks

>try to set width.

353, 437 (important sharp peaks record)


At final timepoint

T29=Myo(:,32);

Looks much different

Fit options, set b1 to term 328

a1 start 5, lower 0, upper 10

>This is fitting it




SO:

Water Raman c term is 5-10 wide Others between 30-50 wide

Five term gaussian to model 5 peaks: 328, 353, 385, 438, 488 35-40 wide c term +/- 5 on all but water raman (+/- 0.5)

So b term gets +/- 5 on the given term at the inf parts The c term is 35-40

Meme dream steelbeams anneliese SCANnoteshit.jpg

Fluorescence Data Analysis

AnnelieseFaustino fluorescencedataanalysis pH9BSAAUNP 161101 Part1.PNG

The chart above shows wavelength of the emission maximums for 3.125 uM BSA, 0.25 mM Au at pH 9 over time. The emission maximum holds relative steady, fluctuating by 5 nm above and below 420 nm for the entire length of the run.

AnnelieseFaustino fluorescencedataanalysis pH9BSAAUNP 161101 Part2.PNG

The chart above shows the integrated emission for 3.125 uM BSA, 0.25 mM Au at pH 9 over time. The integrated emission intensity begins at about 8000 at the start of the run. The integrated intensity increases to 11,500 at around 1200 s (forming a peak). The integrated emission intensity then decreases until about 5500 s (to a value of about 8500 intensity units), when it slowly begins to increase up to 11,000 intensity units until the end of the run at 12,000 s.

AnnelieseFaustino fluorescencedataanalysis pH9BSAAUNP 161101 Part3.PNG

The above chart shows the first 1000 s of emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. The emission intensity is seen to increase rapidly at first, then less slowly over time.

AnnelieseFaustino fluorescencedataanalysis pH9BSAAUNP 161101 Part4.PNG

The above chart shows 1000 s.to 4500 s. of emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. The emission intensity is seen to not increase much more after 1000 s.


AnnelieseFaustino fluorescencedataanalysis pH9BSAAUNP 161101 Part5.PNG


The above chart shows 4500 s to the end of the run of the emission intensity vs. wavelength for 3.125 uM BSA, 0.25 mM Au at pH 9. The water raman peak is pronounced in all scans. Again, the emission intensity is seen to not increase much more after 1000 s.