User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/05

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Sample Preparation

  • Samples were not run with the heater turned on on 161005. The samples were redone on 161006.

BSA Ocean Optics Final Sample Solution Concentrations:

  1. 3 mL total
  2. 0.25 mM Au
  3. 3.125 μM BSA
  4. HCl or NaOH appropriate for your pH (7 today)

Stock solution Concentrations:

  1. 3.73 mM AuCl3
  2. 46.5 mM BSA
  3. 1 mM HCl
  4. DI HOH

Solution Transfer Volumes:

  1. 2597 uL HOH
  2. 201.6 uL AuCl3
  3. 201.0 uL BSA
  4. 0.3 uL HCl

Results

Oceanoptics161006pH7BSA.PNG

The above chart shows the UV-Vis maximum absorbance over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past.

Oceanoptics161006pH7BSAA530.PNG

The above chart shows the UV-Vis A(535.15) over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past, but there is still a trend showing an increase over time.

Oceanoptics161006pH7BSAWAVELENGTH.PNG

The chart above shows an increase in the maximum wavelength around 7000 sec. The readings making up this chart are much "shakier" than previous runs.

  • We may need to redo this run.