User:Anh Do/Notebook/CHEM 481 Polymer/2016/09/02

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

PVOH Film

  • Ingredients
    • 1 g PVOH + 0.5 mL 8% glutaraldehyde in 12 mL water
    • 1 g PVOH + 0.1 g bentonite powder + 0.5 mL glutaraldehyde in 12 mL water
    • 1 g PVOH + variable amount of a crosslinking agent (either equivalent moles of glyoxal or a different quantity of glutaraldehyde) w/o clay
  • Stir at 300 rpm and heat at 70 degree celsius until PVOH and bentonite totally dissolved in the solution
  • Pour the solution to a Teflon disk
  • Films made:

1. PVOH + Glutaraldehyde

  • 1 g PVOH + 0.5 mL 8% glutaraldehyde
    • Mass of PVOH measured: 1.0040 g

2. PVOH + Clay + Glutaraldehyde

  • 1 g PVOH + 0.1 g bentonite powder + 0.5 mL glutaraldehyde

3. PVOH + Clay + Glyoxal

  • 1 g PVOH + 0.1 g bentonite powder + 0.5 mL glutaraldehyde + 0.0553 mL glyoxal
    • Mass of PVOH meassured: 1.0060 g
    • Mass of Bentonite meassured: 0.1001 g
    • Volumn of glyoxal meassured: 0.0553 mL
      • Density of glutaraldehyde = 1.06 g/mL
      • Molar mass of glutaraldehyde = 100.117 g/mol
      • Amount of 8% glutaraldehyde in 0.5 mL = 0.5*(1.06/100.117)*0.08 = 4.235*10^-4 mol
      • Amount of glyoxal needed = 4.235*10^-4 mol
      • Density of glyoxal = 1.27 g/mL
      • Molar mass of glyoxal = 58.04 g/mol
      • Volumn of glyoxal needed = (4.235*10^-4)/(1.27/58.04)/0.35 = 0.0553 mL

Prepare stock solutions

1. 100 mL 200 ppm Malachite Green

  • Add 0.02 g of MG to 100 mL of DI water
    • 200ppm = 200 mg/L
    • Mass of MG powder: 100mL*1L/1000mL*200mg/1L = 20mg
      • Mass of MG measured: 0.02003g

2. 100 mL 3 wt% bentonite

  • Add 3g bentonite to 97mL of water
    • Density of water is 1g/mL
    • 100mL* 1g/mL = 100g
    • 3% of 100g is 3g
      • Measured mass of bentonite:

100mL of 3%wt NaMT

  • 3g NaMT added to 97mL water
    • Measured weight of NaMT:

3. 200 mL water + 40 g Na2SO4 + 4 mL H2SO4 (conc.)

  • Put chemicals together and stir

4. 250 mL 1.0 M HCl solution

  • Add 20mL of conc. HCl to 230 mL of deionized water
    • 12.1 M HCl (conc.)
    • M1V1=M2V2 => 12.1 M HCl * V1 = 1.0 M * 250mL => V1 = 20.8 mL

5. 250 mL 0.2 M sodium bicarbonate solution

  • Add 4.2g NaHCO3 to 250mL water
    • Mr of NaHCO3: 84.007 g/mol
    • Amount of NaHCO3: 250mL * 1L/1000mL*0.2 mol/L= 0.05 mol NaHCO3
    • Mass of NaHCO3: 0.05 NaHCO3 * 84.007 g/mol = 4.2 g NaHCO3
      • Measured mass of NaHCO3:

Pipettes Accuracy Test

  • Glass vial and cap were weighed
  • p-200 pipette was set to 200µL.
  • 200µL of distilled water were pipetted and placed in vial. Vial and water was measured. This step was repeated six times for six different measurements.
  • Data (table made by Dominique)
Pipette Accuracy Data
Pipette Mass of water + Vial + Previous Water Mass (g) Mass of water (g)
p-10 (5 measures)

Mass of Vial: 14.86452g Done by Sarah

14.91830 0.05378
14.9664 0.04814
15.01317 0.04673
15.06537 0.05220
15.12257 0.05720
15.16976 0.04719
p-200

Mass of Vial: 14.98395g

15.18007 0.19612
15.38455 0.020448
15.58249 0.19794
15.78102 0.19853
15.97933 0.19831
16.16458 0.18525
p-1000

Mass of Vial: 14.88254g Done by Anh

16.01650 1.13396
16.98485 0.96835
17.95782 0.97297
18.95590 0.99808
19.94660 0.99070
20.94070 0.99410

Conductivity and pH of water

  • A vial of each kind of water was filled with water and the pH and Conductivity meters were used to measure the pH and conductivity of each.
  • Data
  • Observation

Fluorescence of Water