User:Andy Maloney/Surface passivation effects on kinesin and microtubules/Notebook entries

• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/24/More on kinesin & microtubule buffers
  • Categories: Kinesin and microtubule basics, Kinesin and microtubules, Surface passivation
  • Description: Found a website talking about caseins.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/06/25/Liposomes and kinesin
  • Categories: AM Engineering, Kinesin and microtubules, Surface passivation
  • Description: Doing calculations on the amount of lipids needed to coat a cover slip. The idea was to use lipids as the passivation for the kinesin and microtubule experiments.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/04/Beginning attempts at surface passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Beginning attempts at passivating surfaces with lipids. Also, my ideas with spin coatings.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/11/Spin coater 3000 Trial 1
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Using the Spin Coater 3000.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/12/Surface passivation trial 2
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Using the Spin Coater 3000 again. While I was able to coat the slips with lipids, I was never able to get a motility assay going.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/17/Surface passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: My Spin Coater 3000 didn't cut the mustard. So, we tried using the Fisher centrifuge to coat slips. We were able to do it but, not in a very effective manner.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/19/Surface passivation experiment
  • Categories: AM Engineering, Kinesin and microtubules, Surface passivation
  • Description: Talking about the whole casein issue and how it doesn't go into solution very easily. I did heat up the solution for the first time and started to question why no one has done this before.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/20/Continued setup for surface passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Attempting different concentrations of rhodamine labeled tubulin for fluorescent GMA. Also, I found a webpage and paper that talks about casein in poop. This stemmed from Koch's comment about my heating of PEM to get whole casein to go into solution.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/27/Tubulin resuspension
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Using kappa casein as the surface passivator. This is before I realized that it doesn't work that well.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/28/Motility assay
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: My definition for a new naming convention for the buffers used in our experiments. Also, direct confirmation of Hancock's paper about the length of microtubules supported with different bovine caseins.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/08/31/Passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Noting my update to the protocol section of KochLab. Also, I lament about my paleness and how overexposure to the sun affects my mental faculties.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/02/Surface passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Question the arose from passivation with kappa casein and with lipids. The lipids don't work and the kappa casein does (sort of as I'll find out later).
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/03
  • Categories: Surface passivation
  • Description: Notes on what the differences are between a casein passivated surface and a lipid passivated one and why the lipid surface doesn't work.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/08/Surface passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Thoughts on surface passivation and the differences between hydrophobic/hydrophilic surfaces. I also used potato starch as a surface passivator for some reason.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/09/Surface passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: More thoughts on if you need to passivate both surfaces of a flow cell. Also, I observed what I called "clumping" of microtubules. In fact, these were Taxol crystals masquerading as microtubule clumps. I won't figure that out till later.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/10/Surface passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Using different cleaning methods to produce flow cells resulted in no motility. I believed the reason there was no motility was due to bad kinesin.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/11/Surface passivation studies
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Slide cleaning and its affects on motility. All experiments stopped working. In hindsight, it was due to a contamination issue.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/09/14/Getting motility to work again
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Still trying to get motility to work again. I bought all new stuff and made all new solutions. This is a reoccurring theme with these assays.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/03/Surface passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: More surface passivation stuff. This time with silica nanoparticles.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/13/Lipid preps
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Surface passivation with lipids still not working.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/14/Lipid surface passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Studies with lipids. I will point out that the lipid surfaces do make microtubules stick similar to poly-L-lysine. But, the antifade is just rotten when using a lipid surface.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/15/Lipid passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: More lipid stuff.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/16/Lipid passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: My first realization that Taxol solutions should not sit around for very long.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/19/Lipids discussion
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Some jibba jabba on lipids.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/28/Lipid comparisons
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Success with motility experiments. Not so much success with lipid trials.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29/Lipid stuff
  • Categories: AM Engineering, Kinesin and microtubules, Surface passivation
  • Description: Lamenting on lipid solubility and my procedures for getting them into solution. Also, more computer issues with the microscope setup.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/12/08/50 Point
  • Categories: Heavy water K&MTs, Kinesin and microtubules, Surface passivation
  • Description: Comparison of whole casein and kappa casein passivation.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/07/Surface passivation idea
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Really starting to look at caseins. Abandoning lipids and looking at sialic acids.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/20/Casein constituents
  • Categories: AM Engineering, Kinesin and microtubules, Surface passivation
  • Description: Received casein constituents in today and made 0.5mg/mL PEM solutions with them in it.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/01/26/Caseins
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: First experiment to compare the different constituents of bovine casein as a surface passivator.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/04/MT speed and passivation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Looking at all the different casein solutions. I believe that at this time I was still putting casein in my motility solution. I do not do this anymore.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/03/17/ReAutomation
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: First look at gliding motility speeds using the different bovine caseins as passivators. Still no temperature stabilization for this data.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Casein assay 2
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Redoing the casein assay from 2010-03-04.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/02/Updated casein data
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: This page shows some updated casein data.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/27/Casein data revisited
  • Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules, Surface passivation
  • Description: Reanalyzing the casein data I took to incorporate the times I took the data at. This shows a temperature affect going on.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/27/Reboot
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Initial test to see if all my new solutions work. No data.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Updated reboot
  • Categories: Kinesin and microtubules, Surface passivation
  • Description: Data from yesterday's reboot. Looking at all new solutions. Whole casein speed data.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Caseins
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Looking at casein passivation with all the new solutions made. Beta, alpha, whole so far. Not looking so good.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/29/Beta casein test
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Trying the beta casein assay without putting it in the freezer and without filtering it and not putting it in the freezer. Guess what, it was the filtering.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/04/Solutions
  • Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Again with the remaking of buffers and solutions.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Solutions
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Finished with remaking solutions.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Casein experiments
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Noting that the temperature I have the objective at is not hot enough to have a stable speed measurement.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Casein studies
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: More casein studies with temperature stabilization. The beta casein assay is still acting "funny", or so I think.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/07/Passivation study
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: More surface passivation studies. I've figured out that the harder it is for a casein constituent to go into solution, the better it acts as a surface passivator. Plus, whole and alpha casein work great. Beta and kappa, not so much. Whole and alpha have motility at the center of the flow cell, beta and kappa has it only near the tape.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/10/Casein study
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Continued experiments using the different caseins as surface passivators.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/11/More caseins
  • Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Taking the last bits of data for the bovine casein comparison experiment.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/Casein study
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Continuing the data taking for my passivation study. I've noticed that kappa and beta casein don't work very well when incubated at room temp. So, I'm going to try the cold thing again.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/13/More casein data taking
  • Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Getting the last data points for the passivation study. Also, dealing with the virtualbox update. And, making a stage micrometer.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/07/Casein data
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Looking at the movies I've made for the passivation study. I'm going to try an catalog everything I see in them.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/17/Gliding motility assay movie
  • Categories: AM Engineering, Kinesin and microtubules, Surface passivation
  • Description: Short cartoon of the gliding motiltiy assay.
• Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/23
  • Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
  • Description: Today I tagged all my notebook entries that pertain to the surface passivation paper.