User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/04/10/Gel electrophoresis

From OpenWetWare
Jump to navigationJump to search

Anthony has GRD $$. So we brainstormed on how we should use it. Getting a gel electrophoresis came up. I contacted the VWR people and they gave me this flier. They also wanted to know specific questions such as:

  • Will we use it for imaging?
    • Steve Koch 14:44, 11 April 2009 (EDT): I don't know what they mean by imaging
  • Will we use it for purification?
  • Will we use it for both imaging and purification?
  • How long are the DNA fragments?
    • Steve Koch 14:44, 11 April 2009 (EDT): 100 to 10,000 basepairs

Anthony also asked whether or not we should get a vertical one.

Koch says

Steve Koch 14:44, 11 April 2009 (EDT): I think we can hold off on the vertical (PAGE) one for now. The questions from VWR are strange. Bio-Rad is another common source of gel electrophoresis systems. If VWR, I think we want:

  • 89032-288 (Mini 7) However Anthony should comment on whether this will work for what he's currently been doing.
    • With enough money, we may want to get more than one, but I don't think will be necessary. Extra trays can be convenient.
  • I think we want the 250V power supply (the more expensive one). The reason is, we may want to do constant power someday.
  • An important missing piece is the UV transilluminator. Nas and I (and maybe others) found some of these from Fisher or elsewhere. Approx. $1,000. I can't find them on VWR right now, and I can't access the private wiki for some reason.