User:Andy Maloney/Kinesin & Microtubule Page/PEM-kCT

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Chemicals

PEM

See above page for more information.

PEM-C

See above page for more information.

Taxol

See above page for more information.

Recipe

Notes

This is a dilution of PEM-C from 0.5 mg/mL to 0.25 mg/mL with the addition of 10 µM Taxol. This solution is used for the motility assay.

I typically will prepare this every time I go to make a motility solution. Mainly because it requires the use of Taxol and I do not want to have any solution that contains Taxol sitting around for very long.